Summary
In isolated helical strips of rat aorta, the influence of a variation of bath-temperature on the passive tension as well as on the force of contraction, induced by norepinephrine (=NE) or potassium-depolarization respectively, was investigated.
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1.
Within the range of 37°C and 15°C the passive tension was diminished reversibly by cooling to an extend of 20 dyne/°C (Fig.1a-d, 4a).
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2.
The NE-induced contraction reached its highest value at 37°C. Between 33.5°C and 15°C the existence of a linear function between temperature and force of contraction could be shown. The contraction was almost abolished at 15°C (Fig.3a). Changing the calcium content of the bath-fluid from 2.4 to 1.1 mM, the slope of the linear regression curve increased significantly (Table 1).
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3.
At 37°C the concentration of NE, necessary for the maximum force of contraction, was 2.4×10−6 g/ml. The peak of the dose-response-curve shifted to lower NE-concentration by cooling the bath-fluid (Fig.2). From 37°C to 25°C there was an exponential function between temperature and that NE-concentration which induced the highest degree of contraction (Fig.3b); e.g., lowering the temperature by each 3.14°C, this maximum effective NE-concentration was halved.
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4.
Increasing the potassium concentration to 136 mM, the dependence on temperature of the force of contraction became smaller than after activation by NE (Fig.4b). The influence of a reduction of the calcium concentration was negligible in these experiments (Table 1).
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5.
Changing the temperature from 20°C to 30°C, there was aQ 10 value of 2.8 for the NE-induced contraction and of 1.23 for the activation by depolarization.
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6.
According to earlier results, the activation of the vascular smooth muscle by depolarization on the one hand and NE on the other hand is discussed on the basis of suggesting different mechanisms.
Zusammenfassung
An isolierten Spiralstreifen der Rattenaorta wurde der Einfluß der Badtemperatur auf die passive Ruhespannung und die maximale, durch Noradrenalin (=NA) bzw. Kalium (=K) induzierte Kontraktionsamplitude untersucht.
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1.
Zwischen 37°C und 15°C verminderte eine Abkühlung den Basaltonus reversibel um jeweils 20 dyn/°C (Abb.1a-d und 4a).
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2.
Die adrenerg ausgelöste Kontraktionsamplitude besaß ein Optimum um 37°C, war unter 33,5°C linear zur Temperatur korreliert und bei 15°C nahezu aufgehoben (Abb.3a). In Ca-armer Lösung (1,1 statt 2,4 mM/l) zeigte sich ein signifikant stärkerer Temperatureinfluß (Tab.1).
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3.
Bei 37°C betrug die zur maximalen adrenergen Aktivierung erforderliche NA-Konzentration 2,4·10−6 g/ml. Sie sank zwischen 37°C und 25°C exponentiell um mehr als eine Zehnerpotenz.
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4.
Die durch 136 mM/l K erzeugte Spannung zeigte nur eine geringe Temperaturabhängigkeit (Abb.4b). Eine Senkung der Ca-Konzentration blieb dabei ohne signifikanten Einfluß (Tab.1).
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5.
Bei einem Temperatursprung von 20°C auf 30°C ergab sich für die adrenerge Aktivierung ein Q10 von 2,8 und für die Depolarisation einQ 10 von 1,23. Dieser Befund spricht in Übereinstimmung mit früheren Ergebnissen für eine unterschiedliche Aktivierung der kontraktilen Elemente.
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Peiper, U., Griebel, L. & Wende, W. Unterschiedliche Temperaturabhängigkeit der Gefäßmuskelkontraktion nach Aktivierung durch Kalium-Depolarisation bzw. Noradrenalin. Pflugers Arch. 324, 67–78 (1971). https://doi.org/10.1007/BF00587797
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DOI: https://doi.org/10.1007/BF00587797