Summary
Sodium, glucose and phosphate reabsorption and excretion have been investigated in totally isolated dog kidneys. The potentiation by furosemide of the natriuresis caused by saline loading of the preparation is quantitatively comparable to that observed in the whole animal; the decrease of proximal reabsorption together with its partial distal compensation correspond primarily to a direct renal effect of blood dilution. Glucose reabsorption is proportional to glomerular filtration rate; it does not differ from the values found in the whole dog. Phosphate reabsorption is proportional to the filtered phosphate load up to a value of the latter of 10 mg P/min/100 g; at higher values a true TmPO4 is reached which is higher than in normal or thyroparathyroidectomized dogs. Blood dilution exhibits no influence on TmG. The influence of blood dilution on fractional P reabsorption depends on the P filtered load. At low values of this load, P reabsorption is not influenced; at high values, reabsorption is significantly reduced by dilution. No relationship is demonstrated between glucose and sodium reabsorption inasmuch as the latter is under direct dependence on blood dilution. Furosemide (10−4 M) induces no significant change in either TmG, P reabsorption or glomerular filtration rate.
Similar content being viewed by others
References
Bowman, R. H., Maack, T.: Functional studies on the isolated perfused rat kidney with particular attention to glucose transport. Abstracts, p. 398. IVth International Congress of Nephrology, Stockholm, 1969.
Brenner, B. M., Falchuk, K. H., Keimowitz, R. I., Berliner, R. W.: The relationship between peritubular capillary protein concentration and fluid reabsorption by the renal proximal tubule. J. clin. Invest.48, 1519–1531 (1969).
Corvilain, J.: Effet de l'hormone de croissance sur l'excrétion rénale des phosphates. Acta clin. belg. Suppl. 3 (1966).
— Abramow, M.: Effect of growth hormone on tubular transport of phosphate in normal and parathyroidectomized dogs. J. clin. Invest.43, 1608–1612 (1964).
Cuypers, Y., Nizet, A., Baerten, A.: Technique pour la perfusion de reins de chien avec du sang hépariné. Arch. int. Physiol.72, 245–255 (1964).
Dirks, I. H., Cirksena, W. J., Berliner, R. W.: The effect of saline infusions on sodium reabsorption by the proximal tubule in the dog. J. clin. Invest.44, 1160–1170 (1965).
———: Micropuncture study of the effect of various diuretics on sodium reabsorption by the proximal tubules of the dog. J. clin. Invest.45, 1875–1885 (1966).
Earley, L. E., Martino, J. A., Friedler, R. M.: Factors affecting sodium reabsorption by the proximal tubule as determined during blockade of distal sodium reabsorption. J. clin. Invest.45, 1668–1684 (1966).
Frick, A.: Parathormone as a mediator of inorganic phosphate diuresis during saline infusion in the rat. Pflügers Arch.325, 1–13 (1971).
Gekle, D., Janovsky, M., Slechtova, R., Martinek, J.: Einfluß der glomerulären Filtrationsrate auf die tubuläre Glucosereabsorption bei Kindern. Klin. Wschr.45, 416–419 (1967).
Handley, C. A., Sigafoos, R. B., La Forge, M.: Proportional changes in renal tubular reabsorption of dextran and excretion ofp-aminohippurate with changes in glomerular filtration. Amer. J. Physiol.159, 175–180 (1949).
Hellman, D., Baird, H.R., Bartter, F. C.: Relationship of maximal tubular phosphate reabsorption to filtration rate in the dog. Amer. J. Physiol.207, 89–96 (1964).
Keyes, J. L., Swanson, R. E.: Dependence of glucose Tm on GFR and tubular volume in the dog kidney. Amer. J. Physiol.221, 1–7 (1971).
Knox, F. G.: Effect of increased proximal delivery on furosemide natriuresis. Amer. J. Physiol.218, 819–823 (1970).
Massry, S. G., Coburn, J. W., Kleeman, C. R.: The influence of extracellular volume expansion on renal phosphate reabsorption in the dog. J. clin. Invest.48, 1237–1245 (1969).
Nizet, A., Baerten, A., Dujardin, A., Thoumsin, H., Thoumsin-Moons, J.: Quantitative influence of non hormonal blood factors in the control of sodium excretion by isolated dog's kidney. Kidney International1, 27–37 (1972).
—, Cuypers, Y., Deetjen, P., Kramer, K.: Functional capacity of isolated dog kidney. Pflügers Arch. ges. Physiol.296, 179–195 (1967).
—, Godon, J. P., Mahieu, P.: Quantitative excretion of sodium and water load by isolated dog kidney: autonomous renal response to blood dilution factors. Pflügers Arch.304, 30–45 (1968).
Robson, A. M., Srivastava, P. L., Bricker, N. S.: The influence of saline loading on renal glucose reabsorption in the rat. J. clin. Invest.47, 329–335 (1968).
Shannon, J. A., Farber, S., Troast, L.: Measurement of glucose Tm in normal dog. Amer. J. Physiol.133, 752–761 (1941).
Spitzer, A. Windhager, E. E.: Effect of peritubular oncotic pressure changes on proximal tubular fluid reabsorption. Amer. J. Physiol.218, 1188–1193 (1970).
Steele, T. H.: Increased urinary phosphate excretion following volume expansion in normal man. Metabolism19, 129–139 (1970).
Suki, W. N., Martinez-Maldonado, M., Rouse, D., Terry, A.: Effect of expansion of extracellular fluid volume on renal phosphate handling. J. clin. Invest.48, 1888–1894 (1969).
Toussaint, Ch.: Contribution à l'étude de l'excrétion des électrolytes chez le Chien. Rev. belge. Path.28, 5–139 (1961).
Van Liew, J. B., Deetjen, P., Boylan, J. W.: Glucose reabsorption in the rat kidney. Dependence on glomerular filtration. Pflügers Arch. ges. Physiol.295, 232–244 (1967).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Nizet, A., Dujardin, A., Thoumsin, H. et al. Excretion and tubular reabsorption of sodium, glucose and phosphate by isolated dog kidneys. Influence of blood dilution. Pflugers Arch. 332, 248–258 (1972). https://doi.org/10.1007/BF00587452
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00587452