Summary
The extracellular space (ECS) of rat jejunum, ileum and colon were determined in “in vivo” condition both by means of a continuous perfusion technique and after ligature of the renal pedicles. As a marker, intravenously injected14C-polyethylenglycol (14C-PEG) was used.
The data indicate that the ECS of rats whose renal pedicles were ligated, increases throughout the experiment, due most probably to a hypertonic expansion of the extracellular space. On the contrary, in the experiments with a continuous perfusion the ECS remains constant throughout the experiment.
Total tissue water does not change in the two types of experiments whereas the intracellular water decreases only if the renal pedicles are ligated.
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Esposito, G., Faelli, A. & Capraro, V. The extracellular space of rat intestine “in vivo”. Pflugers Arch. 331, 70–76 (1972). https://doi.org/10.1007/BF00587192
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DOI: https://doi.org/10.1007/BF00587192