Skip to main content
SpringerLink
Log in

The fate of radioiodinated sheep-growth hormone in intact and nephrectomized sheep

  • Published:
Pflügers Archiv Aims and scope Submit manuscript

Summary

The metabolic clearance rate of125I-labelled sheep growth hormone (GH) in intact sheep was calculated to be 61±7.8 l/24 h and in nephrectomized sheep 26±3.9 l/24 h. From these figures a production rate of GH of 122 μg/24 h was derived for intact sheep.

After a single intravenous injection of labelled GH the kidneys extracted more than 90% within 4 h. The extracted GH was metabolized by the kidney, only a small amount (5%) of the radioactivity excreted in the urine could be precipitated by perchloric acid. Renal metabolism of GH was also indicated by the appearance in renal venous blood of labelled material that was not immunoprecipitable. The liver also removed labelled GH and degraded it.

Four hours after the injection of labelled GH most of the radioactivity was in blood and muscle, but there were significant amounts in kidney, liver and fat. Radioactivity that could be precipitated with GH-antiserum was found only in the kidney, thyroid and blood. A high percentage of activity extracted from all these tissues could still be precipitated by perchloric acid.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Andrews, P., Folley, S. J.: Molecular weights of bovine ovine and porcine pituitary growth hormone estimated by gel filtration on Sephadex. Biochem. J.87, 3P (1963).

    Google Scholar 

  2. Bassett, J. M., Thorburn, G. D., Wallace, A. L. C.: The plasma growth hormone concentration of the foetal lamb. J. Endocr.48, 251–263 (1970).

    Google Scholar 

  3. Cameron, D. P., Burger, H. G., Catt, K. J., Doig, A.: Metabolic clearance rate of radioiodinated human growth hormone in man. J. clin. Invest.48, 1600–1608 (1969).

    Google Scholar 

  4. Chamberlain, M. J., Stimmler, L.: The renal handling of insulin. J. clin. Invest.46, 911–919 (1967).

    Google Scholar 

  5. Collip, P. J., Patrick, J. R., Goodheart, C., Kaplan, S. A.: Distribution of tritium labelled human growth hormone in rats and guinea pigs. Proc. Soc. exp. Biol. (N. Y.)121, 173–177 (1966).

    Google Scholar 

  6. Elgee, N. J., Williams, R. H., Lee, N. D.: Distribution and degradation studies with insulin-131I. J. clin. Invest.33, 1252–1260 (1954).

    Google Scholar 

  7. Evans, H. M., Simpson, M. E., Marx, W., Kibrick, E.: Bioassay of the pituitary growth hormone: Width of the proximal epiphyseal cartilage of the tibia of hypophysectomized rats. Endocrinology32, 13–16 (1943).

    Google Scholar 

  8. Finster, J. L., Taylor, A. L., Mintz, D. H.: Metabolic clearance and production rates of human growth hormone. J. clin. Invest.47, 35a (1968).

    Google Scholar 

  9. Frohman, L. A., Bernadis, L. L.: Growth hormone secretion in the rat: metabolic clearance and secretion rates. Endocrinology86, 305–312 (1970).

    Google Scholar 

  10. Gelotte, B. J.: Studies on gel filtration. Sorption properties of the bed material Sephadex. J. Chromatogr.3, 330–342 (1960).

    Google Scholar 

  11. Gharaybeh, H. R., Arnold, G. W., McManus, W. R., Dudzinski, M. L.: Prediction of body composition in live animals. Proc. Aust. Soc. Anim. Prod.VI, 284–290 (1966).

    Google Scholar 

  12. Girard, J., Greenwood, F. C.: The absence of intact growth hormone in urine as judged by radioimmunoassay. J. Endocr.40, 493–503 (1968).

    Google Scholar 

  13. Greenwood, F. C., Hunter, W. M., Glover, J. S.: The preparation of131I-labelled human growth hormone of high specific radioactivity. Biochem. J.89, 114–123 (1963).

    Google Scholar 

  14. Harvey, R. B., Brothers, A. J.: Renal extraction of para-aminohippurate and creatinine measured by continuousin vivo sampling of arterial and renal-vein blood. Ann. N. Y. Acad. Sci.102, 46–54 (1962).

    Google Scholar 

  15. Kohler, P. O., Ross, G. T., Odell, W. D.: Metabolic clearance and production rates of human luteinizing hormone in pre- and post menopausal women. J. clin. Invest.47, 38–47 (1968).

    Google Scholar 

  16. Lee, N. D.: Studies on insulin labelled with131I. Ann. N. Y. Acad. Sci.70, 94–108 (1957).

    Google Scholar 

  17. McCormick, J. R., Sonksen, P. H., Soeldner, J. S., Egdahl, R. H.: Renal handling of insulin and growth hormone in hemorrhagic shock. Surgery66, 175–180 (1969).

    Google Scholar 

  18. Mirsky, I. A., Broh-Kahn, R. H.: The inactivation of insulin by tissue extract. 1. The distribution and properties of insulin inactivating extracts (Insulinase). Arch. Biochem.20, 1–14 (1949).

    Google Scholar 

  19. Odell, W. D., Utiger, R. D., Wilber, F. J., Condliffe, P. G.: Estimation of the secretion rate of thyrotropin in man. J. clin. Invest.46, 953–959 (1967).

    Google Scholar 

  20. Ricketts, H. T., Wildberger, H. L., Regut, L.: The role of the kidneys in the disposal of insulin in rats. Diabetes12, 155–161 (1963).

    Google Scholar 

  21. Rubenstein, A. H., Spitz, I.: Role of the kidney in insulin metabolism and excretion. Diabetes17, 161–169 (1968).

    Google Scholar 

  22. Salmon, S., Utiger, R., Parker, M., Reichlin, S.: The fate of I131-labelled human growth hormone in the rabbit. Endocrinology70, 459–464 (1962).

    Google Scholar 

  23. Smith, H. W.: Principles of renal physiology, p. 212. Oxford: University Press 1956.

    Google Scholar 

  24. Sonnenberg, M., Money, W. L., Dorans, J. F., Lucas, V., Bourque, L.: The distribution of radioactivity in the tissues of the rat after the administration of radioactive growth hormone preparations. Endocrinology55, 709–720 (1954).

    Google Scholar 

  25. Tait, J. F., Burnstein, S.:In vivo studies of steroid dynamics in man. In: Hormones, vol. 5, p. 441. Eds. G. Pincus, K. V. Thimann and E. B. Astwood. New York: Academic Press 1964.

    Google Scholar 

  26. Taylor, M. G., Ullmann, E.: Glomerular filtration after obstruction of the ureter. J. Physiol. (Lond.)157, 38–63 (1961).

    Google Scholar 

  27. Thorburn, G. D., Nicol, D. H., Wallace, A. L. C.: Effect of bilateral nephroctomy of the foetal lamb on growth and development. Proc. endocr. Soc. Aust.13, Abstr. 1 (1970).

    Google Scholar 

  28. Vilar, O., Alverez, B., Davidson, O., Mancini, R. E.: Incorporation by the kidney of fluorescent pituitary hormones. J. Histochem. Cytochem.12, 621–627 (1964).

    Google Scholar 

  29. Wallace, A. L. C., Bassett, J. M.: Plasma growth hormone concentrations in sheep measured by radioimmunoassay. J. Endocr.47, 21–36 (1970).

    Google Scholar 

  30. —, Ferguson, K. A.: The preparation of sheep growth hormone. J. Endocr.26, 259–263 (1963).

    Google Scholar 

  31. Wallace, A. L. C., Stacy, B. D., Thorburn, G. D.: The effect of kidney ligation on the release of plasma free fatty acids following the injection of growth hormone. J. Endocr.48, 297–298 (1970).

    Google Scholar 

  32. Williams, R. H., Hay, J. S., Tjaden, M. B.: Degradation of insulin-131I and glucagon-131I and factors influencing it. Ann. N. Y. Acad. Sci.74, 513–529 (1959).

    Google Scholar 

  33. Zaharko, D. S., Beck, L. V., Blankenbaker, R.: Role of the kidney in the disposal of radioiodinated and non radioiodinated insulin in dogs. Diabetes15, 680–685 (1966).

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Division of Animal Physiology, C.S.I.R.O. Ian Clunies Ross Animal Research Laboratory, 2149, Prospect, N.S.W., Australia

    A. L. C. Wallace, B. D. Stacy & G. D. Thorburn

Authors
  1. A. L. C. Wallace
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. B. D. Stacy
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. G. D. Thorburn
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Wallace, A.L.C., Stacy, B.D. & Thorburn, G.D. The fate of radioiodinated sheep-growth hormone in intact and nephrectomized sheep. Pflugers Arch. 331, 25–37 (1972). https://doi.org/10.1007/BF00587188

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00587188

Key words

Search

Navigation