Summary
An enzyme-kinetic method for the determination of renin activity in rat kidneys is described. Substrate (angiotensinogen) was prepared from plasma of rats nephrectomized 24 h previously. Under the existing conditions, the angiotensin formed during incubation was not inactivated by peptidases, and added angiotensin II-amide was completely recovered. The optimum pH for the reaction of renin extracted from rat kidneys with homologous plasma substrate was found to be 7.2 For the rate of angiotensin formation, aK m of 2,400 ng angiotensin per ml was determined at pH 7.4 and 37°C. The renin activity in kidneys of normal adult rats corresponded to about 10 Goldblatt units of hog renin (NBC) under the same conditions of incubation.
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Orth, H., Krahé, P., Steigelmann, C. et al. An improved method for the determination of renin in rat kidneys. Pflugers Arch. 329, 125–135 (1971). https://doi.org/10.1007/BF00586987
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DOI: https://doi.org/10.1007/BF00586987