Summary
The effect of muscle contraction on lymphatic and plasma [K+], [Na+], [Ca2+], [Mg2+], [Cl−], [Pi], [lactate] ([Lac−]); [creatine] ([Cr]), ideal osmolality (OSM), and [protein] was evaluated in femoral venous blood and lymph specimens sampled from the calf muscles of rabbits before, in the course of, and after contractions. In addition, total [K+], [Na+], [Ca2+], [Mg2+], [Cl−], and [H2O] were analyzed in the muscle tissue. To facilitate lymph sampling both hind limbs were passively flexed and extended, in imitation of natural running movements, by an electrically driven crank. The muscles of one side also performed superimposed rhythmic isotonic contractions. Before contractions, lymphatic [K+], [Na+], [Ca2+], [Mg2+], [Lac−], [Cr], and OSM did not significantly differ from corresponding femoral venous concentrations, [Cl−], and [Pi] were significantly higher, [protein] significantly lower in the lymph than in the plasma. During contractions lymphatic [K+], OSM, [Lac−], and [Pi] were raised significantly more in the lymph compared with the plasma concentrations. [Na+], [Cl−], [Ca2+], and [Mg2+] showed only small changes in the course of contractions and thereafter, and they were altered in a similar way in the lymph and plasma. It was suggested that lymphatic and interstitial concentrations were in equilibrium. Comparing inactive with active muscles, the latter lost K+ but gained Na+, Cl−, and H2O, whereas minimal changes occurred in the [Ca2+] and [Mg2+]. The changes were discussed in connection with the hypothesis that electrolyte shifts might be involved in the activation of the muscular non-proprioceptive interstitial nerve endings which appear to play a role in reflexogenic cardiovascular and respiratory control.
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References
Areskog, N.-H., Arturson, G., Grotte, G.: Heart lymph: Electrolyte composition and changes induced by cardiac glycosides. Biochem. Pharmacol.14, 783–787 (1965)
Bach, C., Lewis, G. P.: Lymph flow and lymph protein concentration in the skin and muscle of the rabbit hind-limb. J. Physiol. (Lond.)235, 477–492 (1973)
Bernt, E., Bergmeyer, H. U., Möllering, H.: Creatin. In: Methoden der enzymatischen Analyse, Vol. II (H. U. Bergmeyer, ed.), pp. 1724–1728. Weinheim/Bergstr.: Verlag Chemie 1970
Casley-Smith, J. R., Florey, H. W.: The structure of normal small lymphatics. Quart. J. exp. Physiol.46, 101–106 (1961)
Conway, E. J.: Nature and significance of concentration relations of potassium and sodium ions in skeletal muscle. Physiol. Rev.37, 84–131 (1957)
Documenta Geigy Wissenschaftliche Tabellen, 6. Auflage. Basel 1960
Gebert, G.: Messung der K+- und Na+-Aktivität mit Mikro-Glaselektroden im Extracellulärraum des Kaninchenmuskels bei Muskelarbeit. Pflügers Arch.331, 204–214 (1972)
Graham, J. A., Lamb, J. F., Linton, A. L.: Measurement of body water and intracellular electrolytes by means of muscle biopsy. Lancet1967 II, 1172–1176
Haddy, F. J., Scott, J. B.: Metabolically linked vasoactive chemicals in local regulation of blood flow. Physiol. Rev.48, 688–707 (1968)
Haljamäe, H., Linde, A., Amundson, B.: Comparative analyses of capsular fluid and interstitial fluid. Amer. J. Physiol.227, 1199–1205 (1974)
Hilton, S. M.: Evidence for inorganic phosphate as the initiator of post-contraction hyperaemia in skeletal muscle. Scand. J. clin. Lab. Invest.29, 135–138 (1972)
Hnik, P., Hudlická, O., Kûcera, J., Payne, R.: Activation of muscle afferents by non-proprioceptive stimuli. Amer. J. Physiol.217, 1451–1457 (1969)
Hník, P., Holas, M., Krekule, I., Kříž, N., Mejsnar, J., Smieško, V., Újec, E., Vyskočil, F.: Work-induced potassium changes in skeletal muscle and effluent venous blood assessed by liquid ion-exchanger microelectrodes. Pflügers Arch.362, 85–94 (1976)
Hohorst, H.-J.: L-(+)-Lactat. Bestimmung mit Lactat-Dehydrogenase und NAD. In: Methoden der enzymatischen Analyse, Vol. II (H. U. Bergmeyer, ed.), pp. 1425–1429. Weinheim/Bergstr.: Verlag Chemie 1970
Kjellmer, I.: The role of potassium ions in exercise hyperaemia. Med. exp. (Basel)5, 56–60 (1961)
Korneliussen, H.: Fenestrated blood capillaries and lymphatic capillaries in rat skeletal muscle. Cell. Tiss. Res.163, 169–174 (1975)
Lasser, R. P., Schoenfeld, M. R., Allen, D. F., Friedberg, C. K.: Reflex circulatory effects elicited by hypertonic and hypotonic solutions injected into femoral and brachial arteries of dogs. Circulat. Res.8, 913–919 (1960)
Leak, V. L.: Studies on the permeability of lymphatic capillaries. J. Cell. Biol.50, 300–323 (1971)
Liu, C. T., Huggins, R. A., Hoff, H. E.: Mechanisms of intraarterial K+-induced cardiovascular and respiratory responses. Amer. J. Physiol.217, 969–973 (1969)
Lundvall, J., Mellander, S., White, T.: Hyperosmolality and vasodilatation in human skeletal muscle. Acta physiol. scand.77, 224–233 (1969)
Manery, J. F.: Water and electrolyte metabolism. Physiol. Rev.54, 334–417 (1954)
Mayerson, H. S., Patterson, R. M., McKee, A., LeBrie, S. J., Mayerson, P.: Permeability of lymphatic vessels. Amer. J. Physiol.203, 98–106 (1962)
McCloskey, D. I., Mitchell, J. H.: Reflex cardiovascular and respiratory responses originating in exercising muscle. J. Physiol. (Lond.)224, 173–186 (1972)
Renkin, E. M.: Transport of potassium-42 from blood to tissue in isolated mammalian skeletal muscles. Amer. J. Physiol.197, 1205–1210 (1959)
Rusznyák, I., Földi, M., Szabó, G.: Lymphologie. Physiologie und Pathologie der Lymphgefäße und des Lymphkreislaufes. 2. Aufl. Stuttgart: G. Fischer 1969
Stacey, M. J.: Free nerve endings in skeletal muscle of the cat. J. Anat. (Lond.)105, 231–254 (1969)
Stegemann, J., Ulmer, H.-V., Böning, D.: Auslösung peripherer neurogener Atmungs- und Kreislaufantriebe durch Erhöhung des CO2-Druckes in größeren Muskelgruppen. Pflügers Arch. ges. Physiol.293, 155–164 (1967)
Steinhagen, C., Hirche, Hj., Hosselmann, I., Manthey, J., Nestle, H. W., Bovenkamp, U.: Interstitial pH of the isolated working skeletal muscle during acid-base disturbances. Pflügers Arch.355, R122 (1975)
Stingl, J., Stembera, O.: Distribution and ultrastructure of the initial lymphatics of some skeletal muscles in the rat. Lymphology7, 159–168 (1974)
Taylor, A. E., Gibson, W. H., Granger, H. J., Guyton, A. C.: The interaction between intracapillary and tissue forces in the overall regulation of interstitial fluid volume. Lymphology6, 192–208 (1973)
Tibes, U., Hemmer, B., Schweigart, U., Böning, D., Fotescu, D.: Exercise acidosis as cause of electrolyte changes in femoral venous blood of trained and untrained man. Pflügers Arch.347, 145–158 (1974)
Tibes, U., Hemmer, B.: Peripheral drive on circulatory and ventilatory centers from muscular metabolic receptors. Pflügers Arch.347, R47 (1974)
Tibes, U., Haberkorn, E., Hemmer, B. with technical assistance of M. Kötter and B. Arndt: Changes of interstitial and venous [K+], [Na+], [Ca2+], [Mg2+], [Cl−], [PO4], osmolality (OSM), [Lactate] ([Lac−]), [creatine] ([Cr]), and muscle electrolytes due to muscular contractions. Pflügers Arch.359, R72 (1975)
Tibes, U., Hemmer, B., Böning, D., Schweigart, U.: Relationships of femoral venous [K+], [H+], PO 2, osmolality, and [orthophosphate] with heart rate, ventilation, and leg blood flow during bicycle exercise in athletes and nonathletes. Europ. J. appl. Physiol.35, 201–214 (1976)
Wildenthal, K., Mierzwiak, D. S., Skinner, N. S. Jr., Mitchell, J. H.: Potassium-induced cardiovascular and ventilatory reflexes from the dog hindlimb. Amer. J. Physiol.215, 542–548 (1968)
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A preliminary report of this work has been given elsewhere [33]
Supported by Deutsche Forschungsgemeinschaft
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Tibes, U., Haberkorn-Butendeich, E. & Hammersen, F. Effect of contraction on lymphatic, venous, and tissue electrolytes and metabolites in rabbit skeletal muscle. Pflugers Arch. 368, 195–202 (1977). https://doi.org/10.1007/BF00585196
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DOI: https://doi.org/10.1007/BF00585196