Summary
Reversible caffeine contractures in isolated muscle fibres of the crayfish (Astacus fluviatilis; m. extensor carpopoditi) are accompanied by swelling of the diadic cisternae of the sarcoplasmic reticulum and by constriction of the T-system tubules. The membranes of the tubules may coalesce and form pentalaminar structures. These changes in the diadic region can be found already in fibres fixed 10 s after the sudden introduction of 4 mM or 6 mM caffeine solution. The changes are completely reversible. The recovery period after a 6 mM caffeine contracture lasts 20 min. The restitution of ultrastructural changes induced by caffeine correlates well with the recovery of responsiveness to a repeated application of caffeine. The pretreatment of fibres with procaine prevents both the generation of caffeine contractures and the occurrence of changes in the ultrastructure. No changes or a very slight enlargement of diadic cisternae were found during potassium contractures of comparable size and duration to caffeine contractures. The effect of procaine as well as the restitution of ultrastructural changes after the recovery of responsiveness to caffeine support the view that the cisternal swelling is a direct consequence of the calcium releasing action of caffeine. Isolated swelling of diadic cisternae and the absence of any changes in the remaining parts of the sarcoplasmic reticulum during reversible caffeine contractures could point to the existence of functional differentiation of the respective parts of the sarcoplasmic reticulum. The absence of ultrastructural changes during potassium contractures might indicate a different source of the activating calcium for contraction and/or a different mechanism of its release.
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References
Axelson, J., Thesleff, S.: Activation of the contractile mechanism in striated muscle. Acta physiol. scand.44, 55–66 (1958)
Bianchi, P. C.: Cell calcium. London: Butterworth 1968
Bittar, E. E., Hift, H., Huddart, H., Tong, E.: The effects of caffeine on sodium transport, membrane potential, mechanical tension and ultrastructure in barnacle muscle fibres. J. Physiol. (Lond.)242, 1–34 (1974)
Brandt, P. W., Reuben, J. P., Girardier, L. Grundfest, H.: Correlated morphological and physiological studies on isolated muscle fibres. I. Fine structure of the crayfish muscle fibre. J. Cell Biol.25, 233–260 (1965)
Caldwell, P. C., Walster, G. E.: Studies on the micro-injection of various substances into crab muscle fibres. J. Physiol. (Lond.)169 353–372 (1963)
Carvalho, A. P.: Calcium-binding properties of sarcoplasmic reticulum as influenced by ATP, caffeine, quinine, and local anaesthetics. J. gen. Physiol.52, 622–642 (1968)
Chiarandini, D. J., Reuben, J. P., Brandt, P. Q., Grundfest, H.: Effects of caffeine on crayfish muscle fibres. I. Activation of contraction and induction of Ca spike electrogenesis. J. gen. Physiol.55, 640–664 (1970a)
Chiarandini, D. J., Reuben, J. P., Girardier, L., Katz, G. M., Grundfest, H.: Effects of caffeine on crayfish muscle fibres. II. Refractoriness and factors influencing recovery (repriming) of contractile responses. J. gen. Physiol.55, 665–687 (1970b)
Feinstein, M. B.: Inhibiton of caffeine rigor and radiocaclcium movements by local anaestheties in frog sartorius muscle. J. gen. Physiol.47, 151–172 (1963)
Fujii, K., Sakai, T.: Electron microscopic studies on “rapid cooling contraction” Jikeikai med. J.16, 75–84 (1969)
Gainer, H.: The role of calcium in excitation-contraction coupling of lobster muscle. J. gen. Physiol.52 88–110 (1968)
Gruener, R.: Caffeine contractures in sarcolemma-free muscle fibres. J. Physiol. (Lond.)191, 106–108 P (1967)
Gutmann, E., Sandow, A.: Caffeine-induced contracture and potentiation of contraction in normal and denervated rat muscle. Life Sci.4 1149–1156 (1965)
Huddart, H., Oates, K.: Localization of the intracellular site of action of caffeine on skeletal muscle. Comp. Biochem. Physiol.36, 677–682 (1970)
Luft, J.: Improvements in epoxy resin embedding methods. J. biophys. biochem. Cytol.9, 409–414 (1961)
Lüttgau, H. C., Oetliker, H.: The action of caffeine on the activation of the contractile mechanism in striated muscle fibres. J. Physiol. (Lond.)194, 51–74 (1968)
Millonig, G.: Advantages of a phosphate buffer for OsO4 solutions in fixation. J. appl. Physiol.32, 1637 (1961)
Peachey, J. D.: Membrane systems of crab fibres. Amer. Zool.7, 505–513 (1967)
Reynolds, E. S.: The use of lead citrate at high pH as an electron microscopy. J. Cell Biol.17, 208–212 (1963)
Sandow, A.: Excitation-contraction coupling in skeletal muscle. Pharmacol. Rev.17, 265–320 (1965)
Selverston, A.: Structure and function of the transverse system in crustacean muscle fibres. Amer. Zool.7, 515–525 (1967)
Thorens, S., Endo, M.: Calcium-induced calcium release and “depolarization”-induced calcium release: Their physiological significance. Proc. Jap. Acad.51, 473–478 (1975)
Uhrík, B., Zacharová, D.: Ultrastructural changes in the internal membraneous system of curstacen muscle fibres during caffeine contracture. Physiol. bohemoslov.17, 496 (1968)
Uhrík, B., Zacharová, D.: The influence of procaine on the caffeine contracture and the ultrastructural appearence of the crayfish muscle fibre. Physiol. bohemoslov.19, 352 (1970)
Uhrík, B., Zacharová, D.: The effect of procaine on the caffeine contracture and ultrastructure of crayfish muscle fibre. Abstr. pap. XXV. Int. Congress Physiol. Sci., p. 1709 (1971)
Valko, L., Zachar, J., Zacharová D.: A mathematical description of the decoupling process in Ca-free medium. Physiol. bohemoslov.16, 208–213 (1967)
Weber, A., Herz, R.: The relationship between caffeine contracture of intact muscle and the effect of caffeine on reticulum. J. gen. Physiol.52, 750–759 (1968)
Zachar, J.: Electrogenesis and contractility in skeletal muscle cells. Baltimore-London: University Park Press 1971
Zachar, J., Zacharová, D.: Potassium contractures in single muscle fibres of the crayfish. J. Physiol. (Lond.)186, 596–618 (1966)
Zacharová, D., Zachar, J.: The effect of external calcium ions on the excitation-contraction coupling in single muscle fibres of the crayfish. Physiol. bohemoslov.16 191–207 (1967)
Zachar, J., Zacharová, D., Henček, M.: Membrane potential of the isolated muscle fibre of the crafish (Astacus fluviatilis) Physiol. bohemoslov.13, 117–128 (1964)
Zacharová, D., Zachar, J., Uhrík, B.: Localization of calcium source and calcium sink in crayfish muscle fibres. Abstr. XXIV. Internat. Congress Physiol. Sci., Washington 1968
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Uhrík, B., Zacharová, D. Recovery of ultrastructural changes accompanying caffeine contractures in isolated muscle fibres of the crayfish. Pflugers Arch. 364, 183–190 (1976). https://doi.org/10.1007/BF00585188
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DOI: https://doi.org/10.1007/BF00585188