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Cycloleucine (1-amino-cyclopentane carboxylic acid): Tubular reabsorption and inhibitory effect on amino acid transport in the rat kidney (microperfusion experiments)

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Summary

Renal tubular reabsorption of cycloleucine (1-amino-cyclopentane carboxylic acid) was studiedin vivo et situ by continuous microperfusion of single proximal tubules of the rat.

The results show:

  1. a)

    cycloleucine is reabsorbed rapidly compared with other amino acids

  2. b)

    this reabsorption is saturable and can be inhibited by oligomycin

  3. c)

    cycloleucine inhibits tubular reabsorption ofl-arginine, glycine, and ofl-phenylalanine. Mutual reciprocal inhibition occurs only withl-phenylalanine (and perhaps also with glycine).

A maximal possible permeability coefficient for cycloleucine (<6·10−5 cm·sec−1) was calculated. Assuming simple 2-parameter kinetics,V max andK m for tubular reabsorption of cycloleucine were estimated.

It can be concluded from the present results that cycloleucine is reabsorbed by a mechanism that transportsl-phenylalanine, but not by the system shared by dibasic amino acids.

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Authors and Affiliations

  1. Physiologisches Institut der Universität Innsbruck/Austria, Innsbruck, Austria

    Stefan Silbernagl

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  1. Stefan Silbernagl
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Additional information

This study was supported by grant Nr. Si 170/2 of the Deutsche Forschungsgemeinschaft and by grant Nr. 1740 of the Austrian “Fonds zur Förderung der Wissenschaftlichen Forschung”.

Parts of this study were reported at the European Colloquium on Renal Physiology held by INSERM at Royaumont/France, 1974 [29].

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Silbernagl, S. Cycloleucine (1-amino-cyclopentane carboxylic acid): Tubular reabsorption and inhibitory effect on amino acid transport in the rat kidney (microperfusion experiments). Pflügers Arch. 353, 241–253 (1975). https://doi.org/10.1007/BF00584287

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  • DOI: https://doi.org/10.1007/BF00584287

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