Summary
To obtain pure culture of epidermal cells from small human biopsies, two different techniques were tested and compared,i.e. separation of epidermis from corium before cultivation by trypsin and suction, and after cultivation by trypsin and collagenase. The most active growth of epidermal cells was obtained by the third technique, since short-term trypsin treatment released only fibroblasts from the culture. Crude collagenase (type I) was less effective than trypsin. Collagenase type II, III and IV had no effect on fibroblast release. Neither trypsin nor collagenases dispersed epidermal cells.
Zusammenfassung
Folgende Methoden wurden, um reine Kulturen von Epidermiszellen kleiner menschlicher Hautstücke zu züchten, geprüft. 1. Zucht der mit Trypsin oder Suktion getrennten epidermalen Schichten. 2. Entfernung begleitender Fibroblasten von den membranös wachsenden Epidermiszellen mit Trypsin oder bakterieller Kollagenase. Die Entfernung von Fibroblasten mit Trypsin gab die besten Resultate, während die Zellen nach kurzer Behandlung mit Trypsin gelöst wurden. Unreine Kollagenase (Typ I) war weniger effektiv als Trypsin. Kollagenasen von Typ II, III und IV verursachten keine Fibroblastenfreisetzung. Weder Trypsin noch Kollagenasen konnten Epidermiszellen ablösen.
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Hentzer, B., Kobayasi, T. Separation of human epidermal cells from fibroblasts in primary skin culture. Arch. Derm. Res. 252, 39–46 (1975). https://doi.org/10.1007/BF00582429
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DOI: https://doi.org/10.1007/BF00582429