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Alinidine modifies the pacemaker current in sheep Purkinje fibers

  • Excitable Tissues and Central Nervous Physiology
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Abstract

(1) The “specific bradycardic agent” alinidine reduces the slope of the diastolic depolarization in sinoatrial tissue and Purkinje fibers. In short Purkinje fibers of sheep, alinidine (28 μM) decreased the pacemaker current by a dual action. The voltage dependence ofi f activation was shifted in the hyperpolarizing direction by 7.8±0.6 mV (n=18,p<0.001) and the conductance of the fully activated\(\bar i_{\text{f}}\) current was reduced to 73±2% (n=18,p<0.001) of its control value. These effects were reversible and dose-dependent. (2) Ionophoretic injections of alinidine caused reversible reductions of the diastolic depolarization rate and simultaneous transient hyperpolarizing shifts of thei f activation range. (3) Some prolongation of the action potential duration was observed at 28 μM and more pronounced at higher concentration. This was presumably the consequence of a reduction by alinidine of outward repolarizing current carried by the background inward rectifier and plateau currenti x. (4) The action of alinidine oni f resulted in a slower activation of a reduced fraction of the pacemaker current at the maximal diastolic potential level. This explains the decrease of the diastolic depolarization rate observed in Purkinje fibers.

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Author notes

  1. Dirk J. Snyders

    Present address: Stahlman Cardiovascular Research Center, Vanderbilt University Medical School, 37232, Nashville, TN, USA

Authors and Affiliations

  1. Laboratory for Electrophysiology, Department of Physiology, University of Antwerp (R.U.C.A.), B-2020, Antwerp, Belgium

    Dirk J. Snyders & Pierre-Paul Van Bogaert

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  1. Dirk J. Snyders
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  2. Pierre-Paul Van Bogaert
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Snyders, D.J., Van Bogaert, PP. Alinidine modifies the pacemaker current in sheep Purkinje fibers. Pflugers Arch. 410, 83–91 (1987). https://doi.org/10.1007/BF00581900

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  • DOI: https://doi.org/10.1007/BF00581900

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