Pflügers Archiv

, Volume 392, Issue 4, pp 372–378 | Cite as

Further evidence for an inverse relationship between macula densa NaCl concentration and filtration rate

  • Josephine Briggs
  • Gisela Schubert
  • Jürgen Schnermann
Transport Processes, Metabolism and Endocrinology; Kidney, Gastrointestinal Tract, and Exocrine Glands

Abstract

It has been concluded that tubulo-glomerular feedback mechanism is triggered by changes in NaCl concentration ([NaCl]) at the macula densa. This conclusion is based on the demonstration that changes in filtration rate produced during retrograde perfusion of the loop of Henle depend upon the perfusate [NaCl]. Experiments were performed to evaluate whether the effect on glomerular function of orthograde perfusion of the loop of Henle is consistent with this conclusion. Early proximal flow rate (\(\dot V_{EP} \)), stop-flow pressure (PSF), early distal chloride concentration ([Cl]), and flow rate were measured during perfusion of the loop of Henle with mannitol solution (300 mosm kg−1), 30 mM NaCl+mannitol (300 mosm kg−1), 140 mM Na isethionate and artificial tubular fluid. When distal flow exceeded 10 nl min−1, the magnitude of the glomerular response was predictable from the [Cl]. The linear regression line,\(\Delta \dot V_{EP} = - 0.027{\text{ }}[Cl]{\text{ + 4}}{\text{.3}}\), did not differ from that obtained previously with the retrograde technique. Retrograde perfusion with 140 mM Na isethionate was without effect on\(\dot V_{EP} \). We conclude that the effect on glomerular function of perfusion of the loop of Henle in either an orthograde or a retrograde direction with these solutions depends upon the chloride concentration at the macula densa.

Key words

Glomerular filtration rate Juxta-glomerular apparatus Macula densa Tubulo-glomerular feedback Chloride 

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References

  1. Bell PD, Thomas C, Williams RH, Navar LG (1978) Filtration rate and stop-flow pressure feedback responses to nephron perfusion in the dog. Am J Physiol 234:F154–165Google Scholar
  2. Bell PD, McLean CB, Navar LG (1979) Distal tubular fluid composition and tubulo-glomerular feedback responses during perfusion with chloride and non-chloride containing solutions. Kidney Int 16:806Google Scholar
  3. Bell PD, Navar LG, Ploth DW, McLean CB (1980) Tubuloglomerular feedback responses during perfusion with nonelectrolyte solutions in the rat. Kidney Int 18:460–471Google Scholar
  4. Bell PD, McLean CB, Navar LG (1981) Dissociation of tubuloglomerular feedback responses from distal tubular chloride concentration in the rat. Am J Physiol 240:F111-F11Google Scholar
  5. Blantz RC, Konnen KS (1977) Relation of distal tubular delivery and reabsorptive rate to nephron filtration. Am J Physiol 233:F315-F324Google Scholar
  6. Briggs JP, Schnermann J, Wright FS (1980) Failure of tubule fluid osmolarity to affect feedback regulation of glomerular filtration. Am J Physiol 239:F427-F432Google Scholar
  7. Good DW, Wright FS (1979) Luminal influences on potassium secretion: sodium concentration and fluid flow rate. Am J Physiol 236:F192-F205Google Scholar
  8. Gutsche HU, Müller-Suur R, Hegel U, Hierholzer K (1980) Electrical conductivity of tubular fluid of the rat nephron. Micropuncture study of the diluting segment in situ. Pflügers Arch 383:113–122Google Scholar
  9. Morgan R, Berliner RW (1969) A study by continuous microperfusion of water and electrolyte movements in the loop of Henle and distal tubule of the rat. Nephron 6:388–405Google Scholar
  10. Müller-Suur R, Gutsche HU (1978) Effect of intratubular substitution of Na and Cl ions on operation of tubuloglomerular feedback. Acta Physiol Scand 103:353–362Google Scholar
  11. Navar LG, Bell PD, Thomas CE, Ploth DW (1978) Influence of perfusate osmolality on stop-flow pressure feedback responses in the dog. Am J Physiol 235:F352-F358Google Scholar
  12. Ramsay JA, Brown RHJ, Groghan PC (1955) Electrometric titration of chloride in small volumes. J Exp Biol 32:822–829Google Scholar
  13. Schnermann J, Briggs J (1981) Concentration dependent NaCl transport as the signal in feedback control of glomerular filtration rate. Kidney Int (in press)Google Scholar
  14. Schnermann J, Hermle M (1975) Maintenance of feedback regulation of filtration dynamics in the absence of divalent cations in the lumen of the distal tubule. Pflügers Arch 358:311–313Google Scholar
  15. Schnermann J, Wright FS, Davis JM, v. Stackelberg W, Grill G (1970) Regulation of superficial nephron filtration rate by tubuloglomerular feedback. Pflügers Arch 318:147–175Google Scholar
  16. Schnermann J, Persson E, Agerup B (1973) Tubulo-glomerular feedback. Nonlinear relation between glomerular hydrostatic pressure and loop of Henle perfusion rate. J Clin Invest 52:862–869Google Scholar
  17. Schnermann J, Ploth DW, Hermle M (1976) Activation of tubuloglomerular feedback by chloride transport. Pflügers Arch 362:229–240Google Scholar
  18. Snedecor GW, Cochran WG (1967) Statistical methods. Ames, Iowa State, pp 153–157Google Scholar
  19. Wahl M, Schnermann J (1969) Microdissection study of the length of different tubular segments of rat superficial nephrons. Z. Anat Entwickl-Gesch 129:128–134Google Scholar
  20. Wright FS, Briggs JP (1979) Feedback control of glomerular blood flow, pressure, and filtration rate. Physiol Rev 59:954–1006Google Scholar
  21. Wright FS, Schnermann J (1974) Interference with feedback control of glomerular filtration rate by furosemide, triflocin and cyanide. J Clin Invest 53:1695–1708Google Scholar

Copyright information

© Springer-Verlag 1982

Authors and Affiliations

  • Josephine Briggs
    • 1
  • Gisela Schubert
    • 1
  • Jürgen Schnermann
    • 1
  1. 1.Physiologisches Institut der Universität MünchenMünchen 2Germany

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