Pflügers Archiv

, Volume 392, Issue 4, pp 372–378 | Cite as

Further evidence for an inverse relationship between macula densa NaCl concentration and filtration rate

  • Josephine Briggs
  • Gisela Schubert
  • Jürgen Schnermann
Transport Processes, Metabolism and Endocrinology; Kidney, Gastrointestinal Tract, and Exocrine Glands


It has been concluded that tubulo-glomerular feedback mechanism is triggered by changes in NaCl concentration ([NaCl]) at the macula densa. This conclusion is based on the demonstration that changes in filtration rate produced during retrograde perfusion of the loop of Henle depend upon the perfusate [NaCl]. Experiments were performed to evaluate whether the effect on glomerular function of orthograde perfusion of the loop of Henle is consistent with this conclusion. Early proximal flow rate (\(\dot V_{EP} \)), stop-flow pressure (PSF), early distal chloride concentration ([Cl]), and flow rate were measured during perfusion of the loop of Henle with mannitol solution (300 mosm kg−1), 30 mM NaCl+mannitol (300 mosm kg−1), 140 mM Na isethionate and artificial tubular fluid. When distal flow exceeded 10 nl min−1, the magnitude of the glomerular response was predictable from the [Cl]. The linear regression line,\(\Delta \dot V_{EP} = - 0.027{\text{ }}[Cl]{\text{ + 4}}{\text{.3}}\), did not differ from that obtained previously with the retrograde technique. Retrograde perfusion with 140 mM Na isethionate was without effect on\(\dot V_{EP} \). We conclude that the effect on glomerular function of perfusion of the loop of Henle in either an orthograde or a retrograde direction with these solutions depends upon the chloride concentration at the macula densa.

Key words

Glomerular filtration rate Juxta-glomerular apparatus Macula densa Tubulo-glomerular feedback Chloride 


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Copyright information

© Springer-Verlag 1982

Authors and Affiliations

  • Josephine Briggs
    • 1
  • Gisela Schubert
    • 1
  • Jürgen Schnermann
    • 1
  1. 1.Physiologisches Institut der Universität MünchenMünchen 2Germany

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