Antonie van Leeuwenhoek

, Volume 59, Issue 3, pp 155–165 | Cite as

Ultrastructure and cell wall composition in cell division cycle mutants ofSchizosaccharomyces pombe deficient in septum formation

  • Pedro Mateos
  • Angel Domínguez
Article
  • 52 Downloads

Abstract

A number of temperature-sensitive cdc- mutants ofSchizosaccharomyces pombe that are affected in septum formation were analyzed with respect to their ultrastructure and the composition of their cell wall polymers. One mutant strain, cdc 16–116, has a cell wall composition similar to the wild type (strain 972 h-). However two other mutants, cdc 4 and cdc 7, show a higher galactomannan content and a lower α-glucan content. In all the mutants tested, total glucose incorporation, protein, RNA and DNA synthesis increased similarly to wild type over 3 1/2 h. After 2–3 h of incubation at the non permissive temperature-35°C-, cell numbers remained constant although, increases in optical densities at 600 nm were observed. According to scanning electron microscopy, the mutants had aberrant shapes after 5h of incubation at 35°C. Transmission electron microscopy showed that cdc 3 is unable to complete septum formation. cdc 4 showed the most varied morphological shapes and aberrant depositions of cell wall material. cdc 8 exhibited a deranged plasma membrane and cell wall regions near of cell poles; an abnormal septum and several nuclei. cdc 7 showed elongated cells with several nuclei and with an apparently normal cell wall completely lacking in septum and septal material. cdc 16 showed more than one septum per cell.

Key words

cell division cycle Schizosaccharomyces pombe cell wall composition septum formation 

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Copyright information

© Kluwer Academic Publishers 1991

Authors and Affiliations

  • Pedro Mateos
    • 1
  • Angel Domínguez
    • 1
  1. 1.Departamento de Microbiología y GenéticaUniversidad de SalamancaSalamancaSpain

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