Skip to main content
SpringerLink
Log in

Isolation and physicochemical characterization of highly polymeric cotton-plant nuclear DNA

  • Published:
Chemistry of Natural Compounds Aims and scope

Abstract

A highly polymeric fraction of nuclear DNA has been isolated from two-day shoots of the cotton plant. The DNA has been characterized by the methods of circular dichroism (CD), thermal denaturation, and ultracentrifugation. It has been established that the DNA preparation isolated has a molecular mass of 18·106 daltons, melts at 85.5°C in a fairly narrow interval, and reveals no heterogeneity of its intramolecular nucleotide composition. It has been shown by the CD method that in a solution of the cotton-plant DNA with relatively low ionic strength there is a tendency to the transformation of the B form into the C form.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Literature cited

  1. J. L. Zimmerman and R. B. Goldberg, Chromosoma,59, 227 (1977).

    Google Scholar 

  2. I. Capesius and J. Meyer, Cytobiologie,15, 485 (1977).

    Google Scholar 

  3. R. Kolodner, K. K. Tewari, and R. C. Warner, Biochim. Biophys. Acta,447, 144 (1976).

    Google Scholar 

  4. A. P. Ibragimov, I. A. Divavin, and Sh. A. Aripdzhanov, Dokl. Akad. Nauk SSSR,192, No. 5, 1164 (1970).

    Google Scholar 

  5. Sh. A. Aripdzhanov, Uzb. Biol. Zh., No. 5, 63 (1974).

  6. I. L. Marmur, Mol. Biol.,3, 208 (1961).

    Google Scholar 

  7. G. E. Sulimova and A. G. Slyusarenko, in: The Structure of DNA and the Position of Organisms in the System [in Russian], Moscow (1972), p. 19.

  8. V. G. Alfrey, V. D. Littay, and A. E. Mirsky, J. Cell. Biol.,21, 213 (1964).

    Google Scholar 

  9. J. Golfrey, Bioph. Chem.,5, 285 (1976).

    Google Scholar 

  10. J. Marmur and P. Doty, J. Mol. Biol.,5, 109 (1962).

    Google Scholar 

  11. J. Brahms, J. Mol. Biol.,11, 785 (1965).

    Google Scholar 

  12. J. Brahms, Proc. Roy. Soc., A,297, 150 (1967).

    Google Scholar 

  13. P. K. Sarkar, B. Wells, and J. T. Jang, J. Mol. Biol.,25, 563 (1967).

    Google Scholar 

  14. P. K. Sarkar and J. T. Jang, in: Conformation of Biopolymers, G. N. Ramachandran, ed., Vol. 1 (1967), p. 197.

  15. V. C. Bode and L. A. Machattie, J. Mol. Biol.,32, 673 (1968).

    Google Scholar 

Download references

Authors
  1. G. F. Kasymova
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. V. K. Burichenko
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. M. Karimov
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

V. I. Nikitin Institute of Chemistry, Academy of Sciences of Tadzhik SSR, Dushanbe, Institute of the Physiology and Biophysics of Plants, Academy of Sciences of the Tadzhik SSR, Dushanbe. Translated from Khimiya Prirodnykh Soedinenii, No. 5, pp. 641–644, September–October, 1984.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kasymova, G.F., Burichenko, V.K. & Karimov, M. Isolation and physicochemical characterization of highly polymeric cotton-plant nuclear DNA. Chem Nat Compd 20, 606–609 (1984). https://doi.org/10.1007/BF00580076

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00580076

Keywords

Search

Navigation