Skip to main content
SpringerLink
Log in

Synthesis of oligodeoxyribonucleotides containing a 3'-terminal phosphate group

  • Published:
Chemistry of Natural Compounds Aims and scope

Abstract

Two methods for synthesizing oligodeoxyribonucleotides containing 3'-terminal phosphosphate groups have been developed. The first consists in the synthesis and the introduction into the oligonucleotides of a block containing a 3'-terminal bis(2-cyanoethyl) phosphate group and the second in the use of the transesterification reaction of oligonucleotides with 2-cyanoethanol in the presence of cesium fluoride. Both methods permit the fairly effective synthesis of oligodeoxyribonucleotide 3'-phosphates.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Literature cited

  1. R. Naylor and P. T. Gilham, Biochemistry,5, 2722 (1966).

    Google Scholar 

  2. Z. A. Shabarova, N. G. Dolinnaya, V. L. Drutsa, N. P. Melnikova, and A. A. Purmal, Nucleic Acids Res.,9, 5747 (1981).

    Google Scholar 

  3. A. G. Badashkeeva, G. N. Kabasheva, D. G. Knorre, G. G. Shamovskii, and T. N. Shubina, Dokl. Akad. Nauk SSSR,206, 870 (1972).

    Google Scholar 

  4. J. Stawinski, T. Hozumi, S. A. Narang, C. P. Bahl, and R. Wu, Nucleic Acid Res.,4, 353 (1977).

    Google Scholar 

  5. Z. A. Shabarova, E. M. Volkov, T. S. Oretskaya, S. I. Turkin, N. G. Dolinnaya, V. K. Karpamanova, and M. A. Prokof'ev, Dokl. Akad. Nauk SSSR,258, 914 (1981).

    Google Scholar 

  6. V. K. Potapov, V. P. Veiko, E. M. Volkov, T. S. Oretskaya, T. R. Telesnina, Z. A. Shabarova, and M. A. Prokof'ev, Dokl. Akad. Nauk SSSR,260, 40 (1981).

    Google Scholar 

  7. G. M. Tener, J. Am. Chem. Soc.,83, 159 (1961).

    Google Scholar 

  8. V. A. Efimov, S. V. Reverdatto, and O. G. Chakhmakhcheva, Tetrahedron Lett.,23, 961 (1982).

    Google Scholar 

  9. J. H. Van Boom and P. H. J. Burgers, Tetrahedron Lett.,52, 4875 (1976).

    Google Scholar 

  10. A. H. Maxam and W. Gilbert, Methods Enzymol.,65, 499 (1980).

    Google Scholar 

  11. V. A. Petrenko, P. I. Pozdnyakov, G. F. Sivolobova, and T. N. Shubina, Bioorg. Khim.,6, 431 (1980).

    Google Scholar 

  12. G. S. Ti, B. L. Gaffney, and R. A. Jones, J. Am. Chem. Soc.,104, 1316 (1982).

    Google Scholar 

  13. R. Crea, A. Kraszewski, T. Hirose, and K. Itakura, Proc. Natl. Acad. Sci. USA,75, 5765 (1978).

    Google Scholar 

Download references

Authors
  1. V. P. Veiko
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. T. S. Oretskaya
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. E. M. Volkov
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. V. G. Metelev
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. E. A. Romanova
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. V. K. Potapov
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

M. V. Lomonosov Moscow State University. Translated from Khimiya Prirodnykh Soedinenii, No. 5, pp. 637–641, September–October, 1984.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Veiko, V.P., Oretskaya, T.S., Volkov, E.M. et al. Synthesis of oligodeoxyribonucleotides containing a 3'-terminal phosphate group. Chem Nat Compd 20, 601–605 (1984). https://doi.org/10.1007/BF00580075

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00580075

Keywords

Search

Navigation