European Biophysics Journal

, Volume 15, Issue 4, pp 193–196 | Cite as

Interaction of proteins with lipid monolayers at the air-solution interface studied by reflection spectroscopy

  • Z. Kozarac
  • A. Dhathathreyan
  • D. Möbius
Article

Abstract

The interaction of a fluorescein-labelled insulin and of cytochrome C with the air-solution interface and with lipid monolayers at the air-solution interface has been studied by measuring the change in surface pressure at constant area and by reflection spectroscopy. Chromophores at the interface only give rise to enhanced light reflection without contribution to the signal from chromophores in the bulk. The accumulation of labelled insulin at the solution surface is very weak as concluded from the shape of the spectrum and reflection intensity. No interaction with a monolayer of dipalmitoyl-phosphatidylcholine at initial surface pressure of 5mN/m was detected. In contrast, the interaction with monolayers of dioctadecyl-dimethyl-ammonium bromide at initial surface pressures between 5 and 40 mN/m is much stronger, leading to a remarkable increase of surface pressure at constant area and strong reflection signal. The technique was also used to detect cytochrome C at the air-solution interface.

Key words

Reflection lipid-protein interaction surface pressure monolayer 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Doty P, Schulman JH (1949) Formation of lipo-protein monolayers. Disc Faraday Soc 6:21–27Google Scholar
  2. Fromherz P (1975) Instrumentation for handling monomolecular films at an air-water interface. Rev Sci Instrum 46:1380–1385Google Scholar
  3. Grüniger H, Möbius D, Meyer H (1983) Enhanced light reflection by dye, monolayers at the air-water interface. J Chem Phys 79:3701–3710Google Scholar
  4. Möbius D, Grüniger H (1984) Organisation of complex monolayers by matrix controlled adsorption. Bioelectrochem Bioenerg 12:375–392Google Scholar
  5. Möbius D, Bücher H, Kuhn H, Sondermann J (1969) Reversible Änderung der Fläche und des Grenzflächenpotentials monomolekularer Filme eines photochromen Systems. Ber Bunsenges Phys Chem 73:845–850Google Scholar
  6. Möbius D, Orrit M, Grüniger H, Meyer H (1985) Orientation of chromophores in monolayers determined from the reflection or transmission of polarised light. Thin Solid Films 132:41–53Google Scholar
  7. Orrit M, Möbius D, Lehmann U, Meyer H (1985) Reflection and transmission of light by dye monolayers. J Chem Phys 85:4966–4979Google Scholar
  8. Quinn PJ, Dawson RMC (1970) The analysis of the interaction of protein with lipid monolayers at the air-water interface. Biochem J 116:671–680Google Scholar
  9. Rietvald A, Berkhout TA, Roenhorst A, Marsh D, Kruijff B (1986) Prelerential association of apocytochrome C with negatively charged phospholipids in, mixed model membranes. Biochim Biophys Acta 858:38–46Google Scholar
  10. Teissie J (1981) Interaction of Cytochrome C with phospholipid monolayers. Orientation and penetration of protein as functions of the packing density of film, nature of the phospho lipids and ionic content of the aqueous phase. Biochemistry 20:1554–1560Google Scholar
  11. Ter-Minassian-Saraga L (1986) Penetration into insoluble monolayers. Langmuir 2:24–29Google Scholar
  12. Verger R, Patus F (1982) Lipid protein interactions. Chem Phys Lipids 30:189–227Google Scholar

Copyright information

© Springer-Verlag 1987

Authors and Affiliations

  • Z. Kozarac
    • 1
  • A. Dhathathreyan
    • 1
  • D. Möbius
    • 1
  1. 1.Max-Planck-Institut für Biophysikalische ChemieGöttingenFederal Republic of Germany

Personalised recommendations