Summary
Viscosities of cell suspensions of human skin epithelium (NCTC strain 2544) were determined by a Wells-Brookfield rotation viscosimeter. A structure viscosity may be postulated by the form of the viscosity curves at different shearrates. At a cell number of 0.5 × 106 cells/ml a viscosity of 1.094 centipoise (25° C, shear-rate 230 s−1) could be found. Since a suspension of single cells may be easily formed mechanically (mopping) the NCTC 2544 cells are useful as investigational model regarding the investigation of membrane characteristics.
Zusammenfassung
Viskositätsmessungen an Zellsuspensionen von menschlichen Hautepithelzellen (Stamm NCTC 2544) wurden unter Verwendung eines Wells-Brookfield Rotationsviskosimeters durchgeführt. Der Kurvenverlauf der Viskositäten bei verschiedenen Scherraten weist auf eine Strukturviskosität hin. Bei einer Zelldichte von 0,5 × 106 Zellen/ml wurde ein Viskositätswert von 1,094 Centipoise (25° C, Scherrate 230 s−1) ermittelt. Da diese Zellen mechanisch (mopping) vereinzelt werden können, eignen sie sich als Modell für Membranuntersuchungen.
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We are grateful to Miss U. Gottschalk for valuable technical assistance with this work
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Neufahrt, A., Förster, F.J., Heine, H. et al. Long-term tissue culture of epithelial-like cells from human skin (NCTC strain 2544). Arch. Derm. Res. 256, 255–260 (1976). https://doi.org/10.1007/BF00572490
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DOI: https://doi.org/10.1007/BF00572490