Summary
The effects of intravenous infusions of adrenaline alone, and after alprenolol (Aptin®) pre-treatment, on whole blood clotting time, factor VIII, fibrinolysis, partial thromboplastin time (PTT), platelet count and fibrinogen have been studied in four healthy, fasting male subjects by double-blind technique using saline infusions as control. The adrenaline infusion caused increases in factor VIII levels, fibrinolysis and platelet counts and a decrease in whole blood clotting time. Alprenolol inhibited the effects of adrenaline on factor VIII and fibrinolysis in all subjects, and the effects on whole blood clotting time in all but one subject. The adrenaline-induced rise in platelet count was not significantly influenced by alprenolol pre-treatment. No effects were seen of the alprenolol infusion itself.
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References
Åblad, B., Brogård, M., Ek, L.: Pharmacological properties of H 56/28 — a β-adrenergic receptor antagonist. Acta pharmacol. (Kbh.)25, suppl. 2, 9–40 (1967).
Bhowal, S.C., Maheshwari, H.B., Kumar, S.: The haematologic functions of lung. II. The role of lung in the release of platelets into circulation. Indian J. Path. Bact.9, 303 (1966).
Bierman, H.R., Kelly, K.H., Cordes, F.L., Byron, R.L., Polhemus, J.A., Rappoport, S.: The release of leukocytes and platelets from the pulmonary circulation by epinephrine. Blood7, 683–692 (1952).
Biggs, R., MacFarlane, R.G., Pilling, J.: Observations on fibrinolysis. Experimental activity produced by exercise or adrenaline. Lancet1947 I, 402–405.
——, Assessment of clotting efficiency. Brit. med. Bull.10, 5–10 (1955).
Blombäck, B.: On the properties of fibrinogen and fibrin. Arkiv Kemi12, 99–113 (1958).
Cannon, W.B., Gray, H.: Factors affecting the coagulation time of blood. II. The hastening or retarding of coagulation by adrenaline injections. Amer. J. Physiol.34, 232–242 (1914).
Cash, J.D., Lind, A.R., McNicol, G.W., Woodfield, D.G.: Fibrinolytic and forearm blood flow responses to intravenous adrenaline in healthy subjects. Life Sci.8, 207–213 (1969).
—— Woodfield, D.G.: Alterations of fibrinolysis and blood coagulation. Lancet1969 I, 54.
Clayton, S., Cross, M.J.: The aggregation of blood platelets by catecholamines and by thrombin. J. Physiol. (Lond.)169, 82–83 (1963).
Cohen, R.J., Epstein, S.E., Cohen, L.S., Dennis, L.H.: Alterations of fibrinolysis and blood coagulation induced by exercise, and the role of beta-adrenergic receptor stimulation. Lancet1968 II, 1264–1266.
Connor, W.E.: The acceleration of thrombus formation by certain fatty acids. J. clin. Invest.41, 1199–1205 (1962).
Egeberg, O.: Changes in the activity of antihemophilic a factor (F. VIII) and in the bleeding time associated with muscular exercise and adrenaline infusion. Scandinav. J. clin. Lab. Invest.15, 539–549 (1963).
Forsberg, S.Å, Johnsson, G.: Hemodynamic effects of propranolol and H 56/28 in man — a comparative study of two β-adrenergic receptor antagonists. Acta pharmacol. (Kbh.)25, suppl. 2, 75–83 (1967).
Forwell, G.D., Ingram, G.I.C.: The effect of adrenaline infusion on human blood coagulation. J. Physiol. (Lond.)135, 371–383 (1957).
Hardisty, R.M., McPherson, J.C.: A one-stage factor VIII (antihaemophilic globulin) assay and its use on venous and capillary plasma. Thrombos. Diathes. haemorrh. (Stuttg.)7, 215–229 (1962).
Holemans, R., Mann, L.S., Mlynarczyk, E.J., Poiesz, B.J.: Drug-induced plasminogen activator release. Thrombos. Diathes. haemorrh. (Stuttg.)18, 298–299 (1967).
Ingram, G.I.C.: Increase in antihaemophilic globulin activity following infusion of adrenaline. J. Physiol. (Lond.)156, 217–224 (1961).
—— Vaughan Jones, R.: The rise in clotting factor VIII induced in man by adrenaline: effect of α- and β-blockers. J. Physiol. (Lond.)187, 447–454 (1966).
—— Knights, S.F., Barrow, E.M., Dejanov, I.I.: The rise in factor VIII concentration after infusion of adrenaline. Brit. J. Haemat.15, 326 (1968).
—— Vaughan Jones, R.: Alterations of fibrinolysis and blood coagulation. Lancet1969 I, 310.
Ishioka, T., Matsumura, T., Shimamoto, T.: Proc. 1st internat. symp. on tissue factors in the homeostasis of the coagulation-fibrinolysis system. Torino: Publ. Unione Chimica Medicamenti 1967.
Korsan-Bengtsen, K., Tibblin, G., Wilhelmsen, L.: Blood coagulation and fibrinolysis in a population study. Abstr. XIII Internat. Congr. of Haemat., Aug. 1970.
Kral, J.G., Åblad, B., Björntorp, P., Ek, L., Johnsson, G.: Metabolic and cardiovascular effects of adrenaline and alprenolol (Aptin®) in human subjects. Pharmacol. Clin.2, 40–45 (1969).
Lee, R.L., White, P.D.: A clinical study of the coagulation time of blood. Amer. J. med. Sci.145, 495–503 (1913).
Libre, E.P., Cowan, D.H., Watkins, S.P., Jr., Shulman, N.R.: Relationships between spleen, platelets and factor VIII levels. Blood31, 358–368 (1968).
Mathur, K.S., Gupta, S.C., Gupta, D.K., Wahal, P.K.: Spontaneous fibrinolysis and the changes produced by adrenaline, fatty meal, storage and diurnal rhythm on fibrinolytic activity. Indian. J. med. Res.52, 38–48 (1964).
McClure, P.D., Ingram, G.I.C., Vaughan Jones, R.: Platelet changes after adrenaline infusions with and without adrenaline blockers. Thrombos. Diathes. haemorrh.13, 136–139 (1965).
Mellander, S.: Comparative studies on the adrenergic neurohormonal control of resistance and capacitance blood vessels in the cat. Acta physiol. scand.50, suppl. 176, 1–86 (1960).
O'Brien, J.R.: Variability in the aggregation of human platelets by adrenaline. Nature202, 1188–1190 (1964).
Poole, J.C.F.: Fats and blood coagulation. Brit. med. Bull.14, 253 (1958).
Rowsell, H.C., Hegardt, B., Downie, H.G., Mustard, J.F., Murphy, E.A.: Adrenaline and experimental thrombosis. Brit. J. Haemat.12, 66–73 (1966).
Schwartz, C.J., Ardlie, N.G.: Catecholamines and platelet aggregation. Circulat. Res.21, suppl. III, 187–201 (1967).
Shimamoto, T., Sunaga, T.: Edematous arterial reaction by adrenaline and cholesterol and its prevention by MAO inhibitor observed by electron microscopic technique. Jap. Heart J.3, 581 (1962).
Todd, A.S.: The histological localisation of fibrinolysin activator. J. Path. Bact.78, 281–283 (1959).
Vendsalu, A.: Studies on adrenaline and noradrenaline in human plasma. Acta physiol. scand.49, suppl. 173 (1960).
Wright, C.-S., Doan, C.A., Bouroncle, B.A., Zollinger, R.M.: Direct splenic arterial and venous blood studies in the hypersplenic syndromes before and after epinephrine. Blood6, 195–212 (1951).
Ygge, J.: Studies on blood coagulation and fibrinolysis in conditions associated with an increased incidence of thrombosis. Scand. J Haemat. 7, suppl. 11 (1970).
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Kral, J.G., Åblad, B., Johnsson, G. et al. Effects of adrenaline and alprenolol (Aptin®) on blood coagulation and fibrinolysis in man. Eur J Clin Pharmacol 3, 144–147 (1971). https://doi.org/10.1007/BF00572454
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DOI: https://doi.org/10.1007/BF00572454