Summary
The relationship between the 8-h time-weighted average (TWA) intensity of exposure toN, N-dimethylformamide (DMF) vapor (with little possibility of skin contact with liquid DMF) and the subsequent excretion ofN-monomethylformamide (MMF) precursor in shift-end urine samples was examined in 116 workers exposed to DMF and 92 workers exposed to DMF in combination with toluene. Urinary MMF level was examined also in 42 non-exposed subjects. The TWA vapor concentration in breathing zone air of each worker was successfully measured by means of a recently developed diffusive sampler in which water was used as an absorbent. The examination of gas chromatographic (GC) conditions for MMF determination showed that the formation of MMF was not saturated when the injection port temperature was set at 200°C, reached a plateau at 250°C, and showed no additional increase at 300°C. There was a linear relationship between DMF in air and MMF in urine with a regression equation ofy =1.65x + 1.69 (r = 0.723,P<0.01), wherey is MMF (unit; mg/l, uncorrected for urine density) in urine andx is DMF (ppm) in air, when only those exposed to DMF were selected, and the injection port temperature was set at 250°C. From this equation, it was possible to estimate that about 10% of the DMF absorbed will be excreted into urine as the MMF precursor. The slope of the regression line was significantly smaller among those exposed to DMF and toluene in combination as compared with those with DMF exposure only.
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References
American Conference of Governmental Industrial Hygienists (1990) The 1990–1991 threshold limit values and biological exposure indices. ACGIH, Cincinnati
Barnes JR, Henry NW III (1974) The determination ofN-methylformamide andN-methylacetamide in urine. Am Ind Hyg Assoc J 35:84–87
Bittersohl G, Berger H (1985) Zur toxikologischen Bedeutung von Dimethylformamid. Z Gesam Hyg 31:345–347
Brindley C, Gescher A, Ross D (1983) Studies of metabolism of dimethylformamide in mice. Chem Biol Interact 45:387–392
Cai S-X, Huang M-Y, Xi L-Q, Li Y-L, Qu J-B, Kawai T, Yasugi T, Mizunuma K, Watanabe T, Ikeda M (1992) Occupational dimethylformamide exposure: 3. Health effects of dimethylformamide after occupational exposure at low concentrations. Int Arch Occup Environ Health 63:461–468
Catenacci G, Ghittori S, Cottica D, Prestinoni A, Capodaglio E (1980) Occupational exposure to dimethylformamide and urinary excretion of monomethylformamide. G Ital Med Lav 2:53–57
Deutsche Forschungsgemeinschaft (1989) Maximum concentrations at the workplace and biological tolerance values for working materials 1989. VCM, Weinheim
Hirayama T, Ikeda M (1979) Applicability of activated carbon felt to the dosimetry of solvent vapor mixture. Am Ind Hyg 40:1091–1096
Inoue O, Seiji K, Kasahara M, Nakatsuka N, Watanabe T, Yin SN, Li G-L, Jin C, Cai S-X, Wang X-Z, Ikeda M (1986) Quantitative relation of urinary phenol levels to breath zone benzene concentrations: a factory survey. Br J Ind Med 43:692–697
Inoue O, Seiji K, Watanabe T, Kasahara M, Nakatsuka H, Yin SN, Li G-L, Cai S-X, Jin C, Ikeda M (1988) Mutual metabolic suppression between benzene and toluene in man. Int Arch Occup Environ Health 60:15–20
Inoue O, Seiji K, Nakatsuka N, Watanabe T, Yin S-N, Li G-L, Cai S-X, Jin C, Ikeda M (1989a) Urinaryt,t-muconic acid as an indicator of exposure to benzene. Br J Ind Med 46:122–127
Inoue O, Seiji K, Kawai T, Jin C, Liu Y-T, Chen Z, Cai S-X, Yin S-N, Li G-L, Nakatsuka H, Watanabe T, Ikeda M (1989b) Relationship between vapor exposure and urinary metabolite excretion among workers exposed to trichloroethylene. Am J Ind Med 15:103–110
Jackson S (1966) Creatinine in urine as an index of urinary excretion rate. Health Phys 12:843–850
Japan Association of Industrial Health (1990) Recommendation for occupational exposure limits (in Japanese). Jpn J Ind Health 32:381–401
Kasahara M, Ikeda M (1987) Spontaneous desorption of organic solvents from carbon cloth. Ind Health 25:73–81
Kawai T, Yasugi T, Mizunuma K, Horiuchi S, Hirase Y, Uchida Y, Ikeda M (1991a) Methanol in urine as a biological indicator of occupational exposure to methanol vapor. Int Arch Occup Environ Health 63:311–318
Kawai T, Mizunuma K, Yasugi T, Horiuchi S, Uchida Y, Iwami O, Iguchi H, Ikeda M (1991b) Urinary methylhippuric acid isomer levels after occupational exposure to xylene isomers. Int Arch Occup Environ Health 63:69–75
Kennedy GL Jr (1986) Biological effects of acetamide, formamide, and their monomethyl and dimethyl derivatives. CRC Crit Rev Toxicol 17:129–182
Kestell P, Gill MH, Threadgill MD, Gescher A, Howarth OW, Curzon EH (1986) Identification by proton NMR ofN-(hydroxymethyl)-N-methylformamide as a major metabolite ofN,N-dimethylformamide in mice. Life Sci 38:719–724
Kimmerle G, Eben A (1975a) Metabolism studies ofN,N′-dimethylformamide. I. Studies in rats and dog. Int Arch Arbeitsmed 34:109–126
Kimmerle G, Eben A (1975b) Metabolism studies ofN,N′-dimethylformamide. II. Studies in persons. Int Arch Arbeitsmed 34:127–136
Krivanek ND, McLaughlin M, Fayerweather WE (1978)N-Methylformamide levels in human urine after repetitive exposure to dimethylformamide vapour. J Occup Med 20:179–182
Lauwerys RR, Kivits A, Lhoir M, Rigolet P, Houbeau D, Buchet JP, Roels HA (1980) Biological surveillance of workers exposed to dimethylformamide and the influence of skin protection on its percutaneous absorption. Int Arch Occup Environ Health 45:189–203
Liu S-J, Qu Q-S, Xu X-P, Liu Y-T, Yin S-N, Takeuchi Y, Watanabe T, Inoue O, Yoshida M, Ikeda M (1992) Toluene vapor exposure and urinary excretion of hippuric acid among toluene exposed workers. Am J Ind Med (in press)
Maxfield ME, Barnes JR, Azar A, Trochimowicz HT (1975) Urinary excretion of metabolites following experimental human exposure to dimethylformamide or dimethylacetamide. J Occup Med 17:506–511
Rainsford SG, Lloyd Davies TA (1965) Urinary excretion of phenol by men exposed to benzene; a screening test. Br J Ind Med 22:21–26
Scailteur V, Hoffman E de, Buchet JP, Lauwerys R (1984) Study on in vivo and in vitro metabolism of dimethylformamide in male and female rats. Toxicology 29:221–234
Scailteur V, Lauwerys RR (1987) Dimethylformamide (DMF) hepatotoxicity. Toxicology 43:231–238
Takada S, Shinoda T, Ohtsuki T, Miyasaka M, Koizumi A, Ikeda M (1983) Comparison between personal and stationary sampling results: a field survey in a printing factory. Bull Environ Contam Toxicol 31:424–427
Uchida Y, Kawai T, Yasugi T, Ikeda M (1990) Personal monitoring sampler for acetone vapor exposure. Bull Environ Contam Toxicol 44:900–904
Ukai H, Takada S, Inui S, Ikeda M (1986) Relationship between exposure and environmental concentration in organic solvent workplaces. Tohoku J Exp Med 149:251–260
Yasugi T, Kawai T, Mizunuma K, Horiguchi S, Ikeda M (1992) Occupational dimethylformamide exposure: 1. Diffusive sampling of dimethylformamide vapor for determination of time-weighted average concentration in air. Int Arch Occup Environ Health 63:449–453
Yonemoto J, Suzuki S (1980) Relation of exposure to dimethylformamide vapour and the metabolite,N-methylformamide, in urine of workers. Int Arch Occup Environ Health 46:159–165
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Kawai, T., Yasugi, T., Mizunuma, K. et al. Occupational dimethylformamide exposure. Int. Arch Occup Environ Heath 63, 455–460 (1992). https://doi.org/10.1007/BF00572111
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DOI: https://doi.org/10.1007/BF00572111