Chemistry of Natural Compounds

, Volume 12, Issue 1, pp 7–9 | Cite as

Determination of uronic acids in natural materials by the decarboxylation method

  • L. I. Glebko
  • G. Ya. Il'chenko
  • L. P. Kosheleva
Article

Summary

A preferred variant of the decarboxylation method for the determination of uronic acids is proposed which uses 57% hydriodic acid and the direct nonaqueous titration of the CO2 formed. The range of concentrations of uronic acids that can be determined is from 2 to 100 µM. The sensitivity of the method is 1.5 µM, its reproducibility 0.6%, and the time of one determination 40–50 min. The proposed variant of the method is applicable to the study of various polysaccharides and other carbohy drate-containing biopolymers.

Keywords

Pectin Uronic Acid Galacturonic Acid Monoethanolamine Decarboxylation Reaction 

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Literature cited

  1. 1.
    K. V. Lefvre and B. Tollens, Ber.,40, 4514 (1907).Google Scholar
  2. 2.
    D. M. W. Anderson, Talanta,2, 73 (1959).CrossRefGoogle Scholar
  3. 3.
    D. M. W. Anderson and S. Garbutt, Talanta,8, 505 (1961).CrossRefGoogle Scholar
  4. 4.
    D. M. W. Anderson, S. Garbutt, and S. S. H. Zaidi, Anal. Chem. Acta,29, 39 (1963).CrossRefGoogle Scholar
  5. 5.
    J. A. Grant, J. A. Hunter, and W. H. S. Massie, Analyst,88, No. 1043, 134 (1963).CrossRefGoogle Scholar
  6. 6.
    P. Braid, J. A. Hunter, W. H. S. Massie, J. D. Nicholson, and B. E. Pearce, Analyst,91, No. 1084, 439 (1966).CrossRefGoogle Scholar
  7. 7.
    M. Bylund and A. Donetzhuber, Svensk. Papperstidn.,71, No. 15, 505 (1968).Google Scholar
  8. 8.
    R. Kaiser, Z. Anal. Chem.,216, No. 1, 80 (1966).CrossRefGoogle Scholar
  9. 9.
    A. P. Kreshkov, Principles of Analytical Chemistry [in Russian], Vol. 2, Moscow (1965), pp. 156–160.Google Scholar
  10. 10.
    A. Steyermark, Quantitative Organic Microanalysis, Blakiston (1951), p. 298.Google Scholar

Copyright information

© Plenum Publishing Corporation 1977

Authors and Affiliations

  • L. I. Glebko
  • G. Ya. Il'chenko
  • L. P. Kosheleva

There are no affiliations available

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