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Der einfluß von autologem serum auf den leukocyten-migrations-inhibitions-test beim melanomalignom

Influence of autologous serum on leucocyte-migration-inhibition-test in melanomalignoma

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Summary

Influence of autologous serum on cellular immunological reactivity to melanomalignoma (MM)-associated antigens was examined on 20 patients having MM at different clinical stages, using the Leucocyte-Migration-Inhibition-Test (MIT). These 20 patients were referring to this till now not investigated.

When heterologous serum (horse serum) was supplemented, an inhibition of cell-migration could be established in 15/20 cases, whereas a cell-migration inhibition in 14/20 cases was absent if autologous serum was added to culture medium.

No correlation could be found between our test results and the clinical stage of the disease.

Our results suggests urgently that blocking factors are present in serum of MM-Patients. There is reason to believe that this factors will be active in lymphocyte level.

Zusammenfassung

Es wurde bei 20 bisher diesbezüglich von uns nicht untersuchten Patienten mit Melanomalignomen (MM) in verschiedenen klinischen Stadien der Einfluß von autologem Serum auf die celluläre Immunreaktivität gegen MM-assoziierte Antigene mit Hilfe des Leukocyten-Migrations-Inhibitions-Testes (MIT) überprüft.

Mit heterologem Serumzusatz (Pferdeserum) wurde in 15/20 Fällen eine Hemmung der Zellwanderung erreicht, während mit autologem Serumzusatz im Kulturmedium eine Hemmung der Zellwanderung bei 14/20 Fällen ausblieb. Die Testergebnisse ließen sich mit den klinischen Stadien des Tumorleidens nicht korrelieren.

Unsere Untersuchungsergebnisse legen dringend nahe, daß im Serum von MM-Patienten blockierende Faktoren vorhanden sind. Es ist anzunehmen, daß diese Faktoren auf lymphocytärer Ebene wirksam werden.

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  1. Universitätsklinik für Dermatologie und Venerologie, Auenbruggerplatz 8, A-8036, Graz, Austria

    J. Fritz & K. Grond

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  1. J. Fritz
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  2. K. Grond
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Fritz, J., Grond, K. Der einfluß von autologem serum auf den leukocyten-migrations-inhibitions-test beim melanomalignom. Arch. Derm. Res. 258, 121–126 (1977). https://doi.org/10.1007/BF00561616

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