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Effect of immunization on the RNA polymerase activity of guinea-pig macrophage nuclei

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Summary

Macrophages from peritoneal exudate contain three types of DNA dependent RNA polymerase. The activity of RNA polymerases in macrophages derived from normal animals is very low. Guinea-pigs were immunized by sheep red blood cells. The immunization enhanced the activity of the RNA polymerase of macrophages derived from peritoneal exudate. The RNA polymerase activity was tested after the solubilization and chromatographic resolution of the three types of polymerases with exogenous template. The results obtained indicated that the immunization enhances the levels of polymerase I and III 10 fold while the level of polymerase II increased 5 fold.

Zusammenfassung

Im peritonealen Exsudat von Meerschweinchen sind drei Typen von DNS-abhängiger RNS-Polymerase nachweisbar. Im Kern der Makrophagen unbehandelter Tiere ist die RNS-Polymeraseaktivität äußerst niedrig. Wir haben Meerschweinchen mit Hammelblutkörperchen immunisiert und die Aktivität der RNS-Polymerase nach vorhergehender Solubilisierung bestimmt. Chromatographisch konnten drei Typen von Polymerase isoliert werden. Auf die Immunisierung hin war der Titer der Polymerasen I und III auf das Zehnfache und jener der Polymerase II auf das Fünffache gestiegen.

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Authors and Affiliations

  1. Department of Dermatology, University Medical School, P.O. Box 480, H-6701, Szeged, Hungary

    Maria Went & Nicholas Simon

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  1. Maria Went
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  2. Nicholas Simon
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Went, M., Simon, N. Effect of immunization on the RNA polymerase activity of guinea-pig macrophage nuclei. Arch. Derm. Res. 255, 259–263 (1976). https://doi.org/10.1007/BF00561496

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  • DOI: https://doi.org/10.1007/BF00561496

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