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Genetics of ocular NAD+-dependent alcohol dehydrogenase and aldehyde dehydrogenase in the mouse: Evidence for genetic identity with stomach isozymes and localization ofAhd-4 on chromosome 11 near trembler

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Abstract

Electrophoretic and activity variation of the stomach and ocular isozyme of aldehyde dehydrogenase (designated AHD-4) was observed between C57BL/6J and SWR/J inbred strains of mice. The phenotypes were inherited in a normal mendelian fashion, with two alleles at a single locus (Ahd-4) showing codominant expression. The alleles assorted independently of those atAdh-3 [encoding the stomach and ocular isozyme of alcohol dehydrogenase (ADH-C2)] on chromosome 3. Three chromosome 11 markers, hemoglobin α-chain (Hba), trembler (Tr), and rex (Re), were used in backcross analyses which established thatAhd-4 is closely linked to trembler. The distribution patterns for stomach and ocular AHD-4 phenotypes were examined among SWXL recombinant inbred mice, and those for stomach and ocular ADH-C2 among BXD recombinant inbred strains. The data provided evidence for the genetic identity of stomach and ocular ADH-C2 and of stomach and ocular AHD-4.

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Author notes

  1. Roger S. Holmes

    Present address: School of Science, Griffith University, Nathan, 4111, Brisbane, Australia

Authors and Affiliations

  1. Department of Genetics, Southwest Foundation for Biomedical Research, P.O. Box 28147, 78284, San Antonio, Texas

    Roger S. Holmes & John L. VandeBerg

  2. Biology Division, Oak Ridge National Laboratory, P.O. Box Y, 37831, Oak Ridge, Tennessee

    Raymond A. Popp

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  1. Roger S. Holmes
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  2. Raymond A. Popp
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  3. John L. VandeBerg
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This research was supported in part by the U.S. Department of Energy under Contract DE-ACO5-84OR214000 with Martin Marietta Energy Systems, Inc. (to R.A.P.).

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Holmes, R.S., Popp, R.A. & VandeBerg, J.L. Genetics of ocular NAD+-dependent alcohol dehydrogenase and aldehyde dehydrogenase in the mouse: Evidence for genetic identity with stomach isozymes and localization ofAhd-4 on chromosome 11 near trembler. Biochem Genet 26, 191–205 (1988). https://doi.org/10.1007/BF00561459

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  • DOI: https://doi.org/10.1007/BF00561459

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