Summary
The human keratinocytes of the outermost granular cell layer were studied by means of conventional electron microscopic procedures to provide more information on the nuclear dense inclusions. The morphology of these inclusions was very variable in respect to their size and density depending on the fixation of specimens for the electron microscopy. The less mature keratinocytes usually contained small inclusion bodies. More mature cells mostly possessed large inclusions. In addition, the cytoplasm of these cells frequently contained similar inclusions. However, cytoplasmic inclusions were absent in keratinocytes lacking similar structures in their nuclei.
Zusammenfassung
Die menschlichen Keratinocyten der äußersten Schicht des Stratum granulosum wurden mit üblichen elektronenmikroskopischen Methoden untersucht, um die Erkenntnisse über die dichten Kerneinschlüsse zu vertiefen.
Es wurde gefunden, daß die Dichte und die Größe dieser Einschlüsse sehr unterschiedlich und von der Fixation abhängig sind. Während die weniger reifen Keratinocyten meistens die kleineren Kerneinschlüsse enthielten, enthielten die reiferen Keratinocyten die größeren Kerneinschlüsse. Keratinocyten mit Kerneinschlüssen enthielten oft ähnliche Einschlüsse auch in ihrem Cytoplasma. Cytoplasmatische Einschlüsse wurden jedoch in Keratinocyten ohne Kerneinschlüsse nicht gefunden.
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With the technical assistance of J. Švihálková and O. Horňák.
The authors thank R. Nettigová for preparing the photocopies.
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Karásek, J., Hrdlička, A. & Smetana, K. Nuclear and cytoplasmic inclusions in normal human epidermis. Arch. Derm. Forsch. 250, 351–357 (1974). https://doi.org/10.1007/BF00558194
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DOI: https://doi.org/10.1007/BF00558194