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Passive release of monoclonal antibodies from hybridoma cells

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Abstract

Evidence is presented for the passive release of monoclonal antibodies (MCAB) from hybridoma cells grown in either batch or continuous-flow culture. This release is promoted at room temperature. Passively released MCAB is indistinguishable from that released by actively growing cells, as judged by SDS-polyacrylamide gel electrophoresis. The significance of these observations in relation to the continuous culture of hybridoma cells is discussed.

Maximum MCAB content of TB/C3 hybridoma cells is about 55pg per cell, any additional MCAB produced is secreted.

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Abbreviations

MCAB:

monoclonal antibodies

PBS:

phosphate buffered saline

RT:

room temperature

SDS:

sodium dodecyl sulphate

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Authors and Affiliations

  1. School of Chemical Engineering, University of Birmingham, P.O. Box 363, B15 2TT, Birmingham, UK

    Sudesh B. Mohan & Andrew Lyddiatt

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  1. Sudesh B. Mohan
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  2. Andrew Lyddiatt
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Mohan, S.B., Lyddiatt, A. Passive release of monoclonal antibodies from hybridoma cells. Cytotechnology 5, 201–209 (1991). https://doi.org/10.1007/BF00556290

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  • DOI: https://doi.org/10.1007/BF00556290

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