Abstract
Hexokinases (EC 2.7.1.1) were genetically analyzed in the mosquitoAedes togoi by agar gel electrophoresis. Enzyme activity was observed anodally in one major banding region (HK-1) on the gel and in another faintly stained region (HK-2). A total of six bands was detected in the HK-1 region. All six bands could be detected in three body parts, head, thorax, and abdomen, of adults with different banding intensities. The third and fourth bands, numbered from the more anodal side, showed the broadest substrate specificity and the greatest enzyme activity throughout development. Genetic analysis of the six HK-1 bands was undertaken on the hypothesis of a single gene locus (or three extremely tightly linked loci). The analysis gave the following gene order:HK-1—4.2±1.8 (recombination units±SE)—To-2—Odh-2—29.5±2.5—sex (M/m)—s. A comparison is made of gene loci for hexokinases among the mosquito speciesCulex pipiens, Aedes aegypti, and this species, along with a comment on linkage relationships betweenHk andOdh (octanol dehydrogenase) loci in threeAedes species.
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References
Ayala, F. J., Powell, J. R., Tracey, M. L., Mourao, C. A., and Perez-Salas, S. (1972). Enzyme variability in theDrosophila willistoni group. IV. Genic variation in natural populations ofDrosophila willistoni.Genetics 70113.
Baker, R. H. (1968). The genetics of “golden,” a new sex-linked color mutant of the mosquitoCulex tritaeniorhynchus Giles.Ann. Trop. Med. Parasitol. 62193.
Barr, A. R., and Myers, C. M. (1966). Two spontaneous mutants ofCulex tarsalis.Proc. Entomol. Soc. Wash. 6849.
Cheng, M.-L., and Hacker, C. S. (1979). The genetics of hexokinase in a mosquito,Culex pipiens.Genetics 92903.
Craig, G. B., Jr., and Hickey, W. A. (1967). Genetics ofAedes aegypti. In Wright, J., and Pal, R. (eds.),Genetics of Insect Vectors of Disease Elsevier Press, Amsterdam. p. 67.
Gilchrist, B. M., and Haldane, J. B. S. (1947). Sex linkage and sex determination in a mosquito,Culex molestus.Hereditas 33175.
Jelnes, J. E. (1971). Identification of hexokinases and localisation of a fructokinase and a tetrazolium oxidase locus inDrosophila melanogaster.Hereditas 67291.
Munstermann, L. E. (1981). Enzyme linkage maps for tracing chromosomal evolution inAedes mosquitoes. In Stock, M. W. (ed.),Application of Genetics and Cytology in Insect Systematics and Evolution University of Idaho, Moscow, p. 129.
Munstermann, L. E., and Craig, G. B., Jr. (1979). Genetics ofAedes aegypti: Updating the linkage map.J. Hered. 70291.
Narang, S., Seawright, J. A., and Joslyn, D. J. (1981). Inheritance and mapping hexokinase-1 and phosphoglucomutase inAnopheles albimanus Wiedemann.Mosq. News 4199.
Pashley, D. P., and Rai, K. S. (1983). Linkage relationships of eleven enzyme loci in theAedes scultellaris group.Biochem. Genet. 211195.
Steiner, W. M. M. (1979). Genetic variation in HawaiianDrosophila. VIII. Heterozygosity and genic changes in isolated populations ofDrosophila engyochracea.Biochem. Genet. 17645.
Tabachnick, W. J. (1978). Identification and linkage relationships of three hexokinase genes inAedes aegypti.Biochem. Genet. 16571.
Tabachnick, W. J., and Howard, D. J. (1982). Genetic control of hexokinase variation in insects.Biochem. Genet. 2047.
Tadano, T. (1977). Genetics of three new mutants, straw-colored larva, ruby eye and pigmented pupa, inAedes (Finlaya) togoi (Diptera: Culicidae).J. Med. Entomol. 1433.
Tadano, T. (1980). Genetics of white eye in the mosquitoAedes togoi.Ann. Trop. Med. Parasitol. 74647.
Tadano, T. (1983). Genetics of octanol and α-glycerophosphate dehydrogenases in the mosquitoAedes (Finlaya) togoi.Biochem. Genet. 211167.
Tadano, T. (1984). A genetic linkage map of the mosquitoAedes togoi.Jpn. J. Genet. 59165.
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This research was supported by National Institutes of Health Grant AI 16983 and a Grant-in-Aid for Co-operative Research (57340033) from the Ministry of Education, Science and Culture, Japan.
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Tadano, T. Genetic studies on hexokinase in the mosquitoAedes togoi . Biochem Genet 25, 375–384 (1987). https://doi.org/10.1007/BF00554546
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DOI: https://doi.org/10.1007/BF00554546