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Electron-cytochemical demonstration of acid phosphatase in saprophytic Claviceps purpurea

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Abstract

p-Nitrophenylphosphate in combination with lead salt technique was used for the cytochemical localization of acid phosphatase (EC 3.1.3.2) in saprophytic submerged culture of Claviceps purpurea Tul. The lead reaction product was found in capsular fibrils, in the newly formed parts of the well wall and in the vacuoles of aged cells (autolysosomes). Phosphatase activity was present also in particulate intracytoplasmatic organelles. The concentric layering of lead deposits in these organelles indicates their relationship to endoplasmic reticulum membranes.

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Abbreviations

pNPP:

p-nitrophenylphosphate

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Authors and Affiliations

  1. Institute of Microbiology, Czechosloyak Academy of Sciences, Budějovická 270, 14220, Praha-4-Krě, ČSSR

    Josef Voříšek

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  1. Josef Voříšek
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Voříšek, J. Electron-cytochemical demonstration of acid phosphatase in saprophytic Claviceps purpurea . Arch. Microbiol. 111, 289–295 (1977). https://doi.org/10.1007/BF00549368

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  • DOI: https://doi.org/10.1007/BF00549368

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