Summary
1. In rats, anaesthetized with urethane, the transfer of tritiated water (HTO) from the blood into the intestinal lumen was investigated by perfusing a jejunal loop in situ.
2. An intravenous infusion of 5-hydroxytryptamine (20 µg/kg·min) or noradrenaline (20 µg/kg·min) decreased the transfer of tritiated water by 33 and 25%.
3. An increased blood volume produced by transfusing blood increased the HTO-transfer whereas a decreased blood volume produced by bleeding decreased the HTO-transfer.
4. The results suggest that changes in the intestinal circulation are responsible for the transfer of HTO from the blood into the intestinal lumen.
5. From the experimental data the unidirectional water flux from the blood into the intestinal lumen was calculated to be 0.93±0.17 µl/min·mg dry weight.
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Literatur
Adolph, E. F., and J. P. Northrop: Absorption of water and chloride (rat). Amer. J. Physiol. 168, 311 (1952).
Bohr, D. F., M. E. Wolf, and P. A. Rondell: Comparison of intravenous and topical effectiveness of various vasoconstrictors on the terminal vascular bed of the rat mesoappendix. Amer. J. Physiol. 182, 311 (1955).
Cater, D. B., S. Garattini, F. Marina, and J. A. Silver: Changes of oxygen tension in brain and somatic tissues induced by vasodilator and vasoconstrictor drugs. Proc. roy. Soc. B 155, 136 (1961).
Code, C. F.: The semantics of the process of absorption. Perspect. Biol. Med. 3, 560 (1960).
Condon, N. E.: A modification of the conventional mercury manometer for blood-pressure recordings. Brit. J. Pharmacol. 6, 19 (1951).
Correll, J. T., L. F. Lyth, S. Long, and J. C. Vanderpoel: Some physiologic responses to 5-hydroxytryptamine creatinine sulfate. Amer. J. Physiol. 169, 537 (1952).
Curran, P. F.: Ion transport in intestine and its coupling to other transport processes. Fed. Proc. 24, 993 (1965).
Durbin, R. P., P. F. Curran, and A. K. Solomon: Ion and water transport in stomach and intestine. Advanc. biol. med. Phys. 6, 1 (1958).
Goodman, L. S., and A. Gilman: The Pharmacological Basis of Therapeutics. New York: The Macmillan Company 1965.
Goodner, C. J., T. E. Moore jr., J. Z. Bowers, and W. D. Armstrong: Effects of acute whole-body X-radiation on the absorption and distribution of Na22 and H3OH from the gastrointestinal tract of the fasted rat. Amer. J. Physiol. 183, 475 (1956).
Haddy, F. J., M. Fleishman, and D. A. Emanuel: The effects of epinephrine, norepinephrine, and serotonin upon systemic small and large vessel resistance. Circulat. Res. 5, 247 (1957).
Hindle, W., and C. F. Code: Some differences between duodenal and ileal sorption. Amer. J. Physiol. 203, 215 (1962).
Johnson, J. A., H. M. Cavert, and N. Lifson: Kinetics concerned with distribution of isotopic water in isolated perfused dog heart and skeletal muscle. Amer. J. Physiol. 171, 687 (1952).
Lecomte, J., et J. Hugnes: Influence de la 5-hydroxytryptamine et de la réserpine sur les réactions vasculaires allergiques du lapin. Int. Arch. Allergy 13, 364 (1958).
Lembeck, F., K.-Fr. Sewing u. D. Winne: Der Einfluß von 5-Hydroxytryptamin auf die Resorption von Tritium-Wasser (HTO) aus dem Dünndarm der Ratte. Naunyn-Schmiedebergs Arch. exp. Path. Pharmak. 247, 100 (1964).
Linder, A.: Statistische Methoden für Naturwissenschafter, Mediziner und Ingenieure. Basel: Birkhäuser 1960.
McGregor, D. D.: The effect of sympathetic nerve stimulation on vasoconstrictor responses in perfused mesenteric blood vessels of the rat. J. Physiol. (Lond.) 177, 21 (1965).
McHardy, G. J. R., and D. S. Parsons: The absorption of water and salt from the small intestine of the rat. Quart. J. exp. Physiol. 42, 33 (1957).
Pinson, E. A.: Water exchanges and barriers as studied by the use of hydrogen isotopes. Physiol. Rev. 32, 123 (1952).
Salmoiraghi, G. C., J. H. Page, and J. W. McCubbin: Cardiovascular and respiratory response to intravenous serotonin in rats. J. Pharmacol. exp. Ther. 118, 477 (1956).
Sewing, K.-Fr., D. Winne u. F. Lembeck: Der Einfluß von 5-Hydroxytryptamin auf die Resorption von Tritium-Wasser (HTO) aus dem Colon der Ratte in vivo. Naunyn-Schmiedebergs Arch. exp. Path. Pharmak. 252, 286 (1965).
Spector, W. G., and D. A. Willoughby: Histamine and 5-hydroxytryptamine in acute experimental pleurisy. J. Path. Bact. 74, 57 (1957).
Spector, W. S.: Handbook of Biological Data. Philadelphia, London: Saunders 1956.
Visscher, M. B., E. S. Fetcher jr., C. W. Carr, H. P. Gregor, M. S. Bushey, and D. E. Barker: Isotopic tracer studies on the movement of water and ions between intestinal lumen and blood. Amer. J. Physiol. 142, 550 (1944).
Weber, E.: Grundriß der biologischen Statistik. Jena: Fischer 1961.
Winne, D.: Der Einfluß einiger Pharmaka auf die Darmdurchblutung und die Resorption tritiummarkierten Wassers aus dem Dünndarm der Ratte. Naunyn-Schmiedebergs Arch. Pharmak. exp. Path. 254, 199 (1966).
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Ochsenfahrt, H., Winne, D., Sewing, KF. et al. Die Ausscheidung von Tritium-Wasser in das Jejunum der Ratte unter dem Einfluß von 5-Hydroxytryptamin und Noradrenalin. Naunyn-Schmiedebergs Arch. Pharmak. u. Exp. Path. 254, 461–469 (1966). https://doi.org/10.1007/BF00539921
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DOI: https://doi.org/10.1007/BF00539921