Summary
A microdetermination of estimating urinary trichloroethanol glucuronide and trichloroacetic acid is described. The glucuronide of trichloroethanol in urine was hydrolysed and oxidized to trichloroacetic acid by chromium trioxide. Trichloroacetic acid thus produced was allowed to react with pyridine and potassium hydroxide in a boiling water bath, and the product was treated with benzidine dihydrochloride or less toxic p-toluidine hydrochloride. In the urine containing trichloroethanol glucuronide and trichloroacetic acid, total trichloro-compound was obtained after oxidation to trichloroacetic acid. The assay of trichloroacetic acid is identical with the above except that digestion step was omitted. And the difference between the two results was considered to estimate the trichloroethanol content.
The analytical sensitivity of this reaction is about 4 times as much as the pyridine potassium hydroxide reaction. Direct microdetermination for urinary trichloroacetic acid is also estimated.
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References
Ahlmark, A., Forssman, S.: Evaluating trichloroethylene exposures by urinalyses for trichloroacetio acid. Arch. industr. Hyg. 3, 386–398 (1951)
Butler, T. C.: Metabolic transformations of trichloroethylene. J. Pharmacol. exp. Ther. 97, 84–92 (1949)
Feigl, F.: Spot tests in organic analysis, Maruzen Asian Edition, pp. 281–282. Amsterdam: Elsevier; Tokyo: Maruzen 1960
Ikeda, M., Ohtsuji, H.: A comparative study of the excretion of Fujiwara reaction-positive substances in urine of humans and rodents given trichloro- or tetrachloro-derivatives of ethane and ethylene. Brit. J. industr. Med. 29, 99–104 (1972)
Leibman, K. C., Hindman, J. D.: Modification of the Fujiwara reaction for determination of polyhalogenated organic compounds. Anal. Chem. 36, 348–351 (1964)
Lugg, G. A.: Fujiwara reaction and determination of carbon tetrachloride, chloroform, tetrachloroethane and trichloroethylene in air. Anal. Chem. 38, 1532–1536 (1966)
Ogata, M., Takatsuka, Y., Tomokuni, K. (with technical assistance of Muroi, K.): A simple method for the quantitative analysis of urinary trichloroethanol and trichloroacetic acid as an index of trichloroethylene exposure. Brit. J. industr. Med. 27, 378–381 (1970)
Ogata, M., Takatsuka, Y., Tomokuni, K.: Excretion of organic chlorine compounds in the urine of persons exposed to vapours of trichloroethylene and tetrachloro-ethylene. Brit. J. industr. Med. 28, 386–391 (1971)
Seto, T. A., Schultze, M. D.: Determination of trichloroethylene, trichloroacetic acid, and trichloroethanol in urine. Anal. Chem. 28, 1625–1629 (1956)
Tada, O., Nakaaki, K., Fukabori, S.: On the methods of determinations of chlorinated hydrocarbons in the air and their metabolites in the urine (in Japanese). J. Sci. Labour 44, 500–516 (1968)
Tanaka, S., Ikeda, M.: A method for determination of trichloroethanol and trichloroacetic acid in urine. Brit. J. industr. Med. 25, 214–219 (1968)
Uebeline, F., Pletscher, A.: Ätiologie und Prophylaxe gewerblicher Tumoren in der Farbstoffindustrie. Schweiz. med. Wschr. 84, 917–920 (1954)
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Ogata, M., Tomokuni, K. & Asahara, H. Simple microdetermination of trichloroethanol glucuronide and trichloroacetic acid in urine. Int. Arch. Arbeitsmed 32, 203–215 (1974). https://doi.org/10.1007/BF00539337
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DOI: https://doi.org/10.1007/BF00539337