Summary
Experiments are described in which the influence of an α-adrenergic blocking agent, phentolamine, and of two hypotensive drugs, diazoxide and 2-(2,6-dichlorophenylamino)-2-imidazoline hydrochloride (DCAI) on blood glucose, plasma insulin and glucose-stimulated insulin secretion has been measured in male Wistar rats.
Blood glucose concentration has been found to be decreased and plasma insulin concentration has been found to be increased after one or two injections of 50 mg phentolamine/kg b.w., intraperitoneally. These results are in accordance with the observation that α-adrenergic stimulation inhibits and β-adrenergic stimulation promotes insulin secretion (Porte, 1967 a, 1967 b). If the α-adrenergic stimulatory effect of endogenous catecholamines is blocked by phentolamine, the remaining β-adrenergic stimulatory effect of endogenous epinephrine leads to an increase in insulin secretion.
Treating rats with diazoxide (200 mg/kg b.w., i.v.) or DCAI (200 μg/kg b.w., s.c.), respectively, has been found to lead to a decrease in plasma insulin concentration as well as to an almost complete inhibition of the ability of the pancreas to secrete insulin in response to an elevation of blood glucose concentration. As these effects have been found to be abolished by pretreatment with phentolamine, the inhibition of insulin secretion caused by diazoxide and DCAI may be regarded as being mediated by α-adrenergic stimulation.
Recent studies of Turtle and Kipnis (1967) have shown that α-adrenergic stimulation lowers islet 3′,5′-AMP concentration and prevents the increase in islet 3′,5′-AMP concentration which is caused by β-adrenergic stimulation. In view of these results indicating an antagonistic influence of α- and β-adrenergic stimulation on 3′,5′-AMP formation in islet tissue, the inhibitory action of diazoxide and DCAI on insulin secretion may be explained by a decrease in adenyl cyclase activity in this tissue.
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Literatur
Bartelheimer, H. K., W. Losert, G. Senft u. R. Sitt: Störungen des Kohlenhydratstoffwechsels im Kaliummangel. Naunyn-Schmiedebergs Arch. Pharmak. exp. Path. 258, 391 (1967).
Blackard, W. G., and Ch. N. Aprill: Mechanism of action of diazoxide. J. Lab. clin. Med. 69, 960 (1967).
Bock, K. D., V. Heimsoth, P. Merguet u. J. Schönermark: Klinische und klinisch-experimentelle Untersuchungen mit einer neuen blutdrucksenkenden Substanz: Dichlorphenylaminoimidazolin. Dtsch. med. Wschr. 91, 1761 (1966).
Bouman, P. R.: Growth hormone and pancreatic beta cell function. J. Endocr. 37, 14 (1967).
Campbell, J., and K. S. Rastogi: Effects of glucagon and epinephrine on serum insulin and insulin secretion in dogs. Endocrinology 79, 830 (1966a).
—— —— Augmented insulin secretion due to growth hormone (stimulatory effects of glucose and food in dogs). Diabetes 15, 749 (1966b).
Coore, H. G., and P. J. Randle: Regulation of insulin secretion studied with pieces of rabbit pancreas incubated in vitro. Biochem. J. 93, 66 (1964).
Frerichs, H., R. Gerber u. W. Creutzfeld: Insulinsekretion in vitro. II. Hemmung der glucoseinduzierten Insulinabgabe durch Diazoxide. Diabetologia 2, 269 (1966).
—— u. W. Creutzfeld: Insulinsekretion in vitro. I. Hemmung der glucoseinduzierten Insulinabgabe durch Insulin. Klin. Wschr. 43, 136 (1965).
Frohman, L. A., M. H. McGillivray, and T. Aceto jr.: Acute effects of human growth hormone on insulin secretion and glucose utilisation in normal and growth hormone deficient subjects. J. clin. Endocr. 27, 561 (1967).
Genuth, S., and H. Lebovitz: Stimulation of insulin release by corticotropin. Endocrinology 76, 1093 (1965).
Grodsky, G. M., L. L. Bennett, D. F. Smith, and F. G. Schmid: Effect of pulse administration of glucose or glucagon on insulin secretion in vitro. Metabolism 16, 222 (1967).
Halpern, B. N., et A. Pacaud: Technique de prélèvement d'èchantillons de sang chez les petits animaux de laboratoire par ponction de plexus ophthalmique. C. R. Soc. Biol. (Paris) 145, 1465 (1951).
Hertellendy, F., L. J. Machelin, R. S. Gordon, M. Horino, and D. M. Kipnis: Lipolytic activity and inhibition of insulin release by epinephrine in the pig. Proc. Soc. exp. Biol. (N. Y.) 121, 675 (1966).
Hoefke, W.: Persönliche Mitteilung, unveröffentlichte Untersuchungen (1967).
——, u. W. Kobinger: Pharmakologische Wirkungen des 2-(2,6-Dichlorphenylamino)-2-imidazolin-hydrochlorids, einer neuen antihypertensiven Substanz. Arzneimittel-Forsch. 16, 1038 (1966).
Huggett, A. S. G., and D. A. Nixon: Use of glucose oxidase, peroxidase, and o-dianisine in determination of blood and urinary glucose. Lancet 1957 II, 368.
Jungas, R. L.: Role of cyclie 3′,5′-AMP in the response of adipose tissue to insulin. Proc. nat. Acad. Sci. (Wash.) 56, 757 (1966).
Kaneto, A., K. Kosaka, and K. Nakao: Effects of the neurohypophysial hormones on insulin secretion. Endocrinology 81, 783 (1967).
Karam, J. H., S. G. Grasso, L. C. Wegienka, G. M. Grodsky, and P. H. Forsham: Effect of selected hexoses, of epinephrine and of glueagon on insulin secretion in man. Diabetes 15, 571 (1966).
Ketterer, A., A. M. Eisentraut, and R. H. Unger: Effect upon insulin secretion of physiologic doses of glucagon administered via the portal vein. Diabetes 16, 283 (1967).
Kris, A. O., R. E. Miller, F. E. Wherry, and J. W. Mason: Inhibition of insulin secretion by infused epinephrine in rhesus monkeys. Endocrinology 78, 87 (1966).
Kühns, K., H. Frisius u. J. Oloffs: Erste klinische Untersuchungen mit einer blutdrucksenkenden Imidazolinverbindung. Dtsch. med. Wschr. 91, 2205 (1966).
Lacy, P. E., and M. Kostianovsky: Method for the isolation of intact islets of Langerhans from the rat pancreas. Diabetes 16, 35 (1967).
Lambert, A. E., B. Jeanrenaud, and A. E. Renold: Enhancement by caffeine of glucagon-induced and tolbutamide-induced insulin release from isolated foetal pancreatic tissue. Lancet 1967 I, 819.
Lebovitz, H. E., and K. Pooler: Puromycin potentiation of corticotropin induced insulin release. Endocrinology 80, 656 (1967).
—— —— ACTH-mediated insulin secretion: effect of aminophylline. Endocrinology 81, 558 (1967a).
Lefebvre, P. J., et A. S. Luyckx: Influence du glucagon sur le débit circulatoire pancréatico-duodénal et sur l'insulinémie du sang veineux pancréatique du chien. Arch. int. Physiol. Biochim. 74, 867 (1966).
Losert, W., G. Senft, R. Sitt, G. Schultz u. H. Kaess: Die Beteiligung des Insulins an der Diazoxid-Hyperglykämie. Naunyn-Schmiedebergs Arch. exp. Path. Pharmak. 253, 388 (1966).
--, u. R. Sitt: Unveröffentlichte Untersuchungen (1968).
Malaisse, W., F. Malaisse-Lagae, E. F. McCraw, and P. H. Wright: Insulin secretion in vitro by pancreatic tissue from normal, adrenalectomized, and cortisol treated rats. Proc. Soc. exp. Biol. (N. Y.) 124, 924 (1967).
—— —— —— Effects of thyroid function upon insulin secretion. Diabetes 16, 643 (1967).
Malaisse, W., F. Malaisse-Lagae, P. H. Wright, and J. Ashmore: Effects of adrenergic and cholinergic agents upon insulin secretion in vitro. Endocrinology 80, 975 (1967).
Mandel, L. R., and F. A. Kuehl jr.: Lipolytic action of 1-triiodothyronine — a cyclic 3′,5′-AMP phosphodiesterase inhibitor. Fed. Proc. 26, 810 (1967).
Marri, G., e G. Cozzolino: Stimulazione della secrezione insulinica nell'uomo mediante infusione protratta di glucagone. Boll. Soc. ital. Biol. sper. 42, 1422 (1967).
Martin, J. H., and J. J. Gagllardino: Effect of growth hormone on the isolated pancreatic islets of rat in vitro. Nature (Lond.) 213, 630 (1967).
Michel, D., W. Zimmermann, A. Nassehi u. P. Seraphim: Erste Beobachtungen über einen antihypertensiven Effekt von 2-(2,6-Dichlorphenylamino)-2-imidazolin-hydrochlorid am Menschen. Dtsch. med. Wschr. 91, 1540 (1966).
Morgan, C. R., and A. Lazarow: Immunoassay of insulin using a two-antibody-system. Proc. Soc. exp. Biol. (N. Y.) 111, 29 (1962).
—— —— Immunoassay of insulin: Two antibody system. Diabetes 12, 115 (1963).
——, and A. Lazarow: Further studies of an inhibitor of the two antibody immunoassay system. Diabetes 13, 579 (1964).
Murad, F., Y.-M. Chi, T. W. Rall, and E. W. Sutherland: Adenyl cyclase. III. The effect of catecholamines and choline esters on the formation of adenosine 3′,5′-phosphate by preparations from cardiac muscle and liver. J. biol. Chem. 237, 1233 (1962).
Orsetti, A., J. Basseres et A. Macabies: Variations de l'insulino-sécrétion au cours de traitements par extraits thyreoidiens chez le chien. J. Physiol. (Paris) 59, 276 (1967).
Porte, D., jr.: A receptor mechanism for the inhibition of insulin release by epinephrine in man. J. clin. Invest. 46, 86 (1967a).
—— Beta adrenergic stimulation of insulin release in man. Diabetes 16, 150 (1967).
—— A. L. Graber, T. Kuzuya, and R. H. Williams: The effect of epinephrine on immunoreactive insulin levels in man. J. clin. Invest. 45, 228 (1966).
——, and R. H. Williams: Inhibition of insulin release by norepinephrine in man. Science 152, 1248 (1966).
Robison, G. A., R. W. Butcher, and E. W. Sutherland: Adenyl cyclase as an adrenergic receptor. Ann. N. Y. Acad. Sci. 139, 703 (1967).
Samols, E., G. Marri, and V. Marks: Interrelationship of glucagon, insulin, and glucose. The insulinogenic effect of glucagon. Diabetes 15, 855 (1966).
Schneider, K. W., u. W. Gattenlöhner: Hämodynamische Untersuchungen nach St 155 [2-(2,6-Dichlorphenylamino)-2-imidazolin-hydrochlorid] beim Menschen. Dtsch. med. Wschr. 91, 1533 (1966).
Schultz, G., u. K. Munske: Unveröffentlichte Untersuchungen (1967).
—— u. R. Sitt: Biochemische Grundlagen der Diazoxid-Hyperglykämie. Naunyn-Schmiedebergs Arch. exp. Path. Pharmak. 253, 372 (1966).
—— —— u. K. Munske: Der Einfluß von Insulin auf die enzymatische Regulation der Glycogenolyse. Naturwissenschaften 53, 529 (1966).
Senft, G.: Beeinflussung hormonaler und enzymatischer Regulationen im Kohlenhydratstoffwechsel bei Anwendung von Benzothiadiazinen. Internist 7, 426 (1966).
—— Hormonal control of carbohydrate and lipid metabolism and drug induced alterations. Naunyn-Schmiedebergs Arch. Pharmak. exp. Path. 259, 117 (1968).
Senft, G., W. Losert, G. Schultz, R. Sitt u. H. K. Bartelheimer: Ursachen der Störungen im Kohlenhydratstoffwechsel unter dem Einfluß sulfonamidierter Diuretica. Naunyn-Schmiedebergs Arch. Pharmak. exp. Path. 255, 369 (1966).
-- G. Schultz, K. Munske, and M. Hoffmann: Influence of insulin on cyclic 3′,5′-AMP phosphodiesterase activity in liver, skeletal muscle, adipose tissue, and kidney (In Vorbereitung) (1968a).
-- -- -- -- Effects of glucocorticoids and insulin on 3′,5′-AMP phosphodiesterase activity in adrenalectomized rats. (In Vorbereitung) (1968b).
Sussmann, K. E., and G. D. Vaughan: Insulin release after ACTH, glucagon, and adenosine-3′,5′-phosphate (cyclic AMP) in the perfused isolated rat pancreas. Diabetes 16, 449 (1967).
Sutherland, E. W., and G. A. Robison: The role of cyclic 3′,5′-AMP in response to catecholamines and other hormones. Pharmacol. Rev. 18, 145 (1966).
Turtle, J. R., and D. M. Kipnis: An adrenergic receptor mechanism for the control of cyclic 3′,5′ adenosine monophosphate synthesis in tissues. Biochem. biophys. Res. Commun. 28, 797 (1967).
——, and D. M. Kipnis: Stimulation of insulin secretion by theophylline. Nature (Lond.) 213, 727 (1967).
Vecchio, D., A. Luyckx, G. R. Zahnd, and A. E. Renold: Insulin release induced by glucagon in organ cultures of fetal rat pancreas. Metabolism 15, 577 (1966).
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Am 31. Oktober 1967 verstorben.
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Senft, G., Sitt, R., Losert, W. et al. Hemmung der Insulininkretion durch α-Receptoren stimulierende Substanzen. Naunyn-Schmiedebergs Arch. Pharmak. u. Exp. Path. 260, 309–323 (1968). https://doi.org/10.1007/BF00537636
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DOI: https://doi.org/10.1007/BF00537636
Key-Words
- Insulin Secretion and its Control
- α-Receptors and Insulin Secretion
- 3′,5′-AMP and Insulin Secretion
- Diazoxide and Insulin Secretion
- 2-(2,6-Dichlorophenylamino)-2-Imidazoline Hydrochloride (DCAI) and Insulin Secretion