Summary
In rats changes in plasma membrane enzyme activities due to Gal-N intoxication were studied by enzymehistochemical methods. The bile canalicular 5′-nucleotidase and nucleoside polyphosphatase activities decreased; the sinusoidal 5′-nucleotidase remained unchanged. The bile canalicular leucyl-β-naphthyl-amindase showed an increase in activity; the alkaline phosphatase activity remained unchanged. In contrast to the spotty necrosis, changes in plasma membrane enzyme activities were seen in all liver cells, suggesting that changes of these activities, occurring after Gal-N treatment, do not correlate with cell death. The conclusion was drawn that the deviations of the enzyme activities might be due to changes in the lipid environment of the enzyme proteins in the membrane.
With the exception of alkaline phosphatase, partial hepatectomy caused the same changes in enzyme activities as did Gal-N intoxication. Nevertheless Gal-N administration to partial hepatectomized rats did not lead to hepatic necrosis. Galactose given simultaneously or within two hours after Gal-N prevented both changes in plasma membrane enzyme activities and hepatocellular damage. This suggests an important role of galactolipids and galactoproteins in the plasma membrane alterations.
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Bachmann, W., Harms, E., Hassels, B., Henninger, H., Reutter, W.: Studies on rat liver plasma membrane. Altered protein and phospholipid metabolism after injection of D-galactosamine. Biochem. J. 166, 455–462 (1977)
Bachmann, W., Reutter, W.: Plasma membrane of the regenerating rat liver plasma membrane as a key target organelle in galactosamine-induced hepatitis. Hoppe-Seyler's Z. Physiol. Chem. 360, 81–87 (1979)
Bauer, C.H., Lukaschek, R., Reutter, W.G.: Studies on the Golgi-apparatus. Cumulative inhibition of protein and glycoprotein secretion by d-galactosamine. Biochem. J. 142, 221–230 (1974)
Decker, K., Keppler, D.: Comparative biochemical studies on acute experimental hepatitis induced by MHV-3 virus and by d-galactosamine. Abstr. 7th Meeting E.A.S.L. (1972)
Decker, K., Keppler, D.: Galactosamine hepatitis: Key-role of the nucleotide deficiency period in the pathogenesis of cell injury and cell death. Rev. Physiol. Biochem. Pharmacol. 71, 78–106 (1974)
Farber, J.L., El-Mofty, S.K.: The biochemical pathology of liver cell necrosis. Am. J. Pathol. 81, 237–250 (1975)
Van Gool, J., Boers, W., De Nie, I.: Inhibitory effect of rat α2 macrofetoprotein (αM FP) an acute phase globulin, on galactosamine hepatitis. Exp. Mol. Pathol. 29, 228–240 (1978)
Grün, M., Luks, H., Rasenack, U.: Significance of endotoxaemia in experimental galactosaminehepatitis in the rat. Acta Hepato-Gastroenterol. 24, 64–81 (1977)
Guilloze, A., Belanger, L., Beaumont, C., Valet, J.P., Briggs, R., Chin, J.F.: Cellular and subcellular immunolocalization of α1-fetoprotein and albumin in rat liver. Reevaluation of various experimental conditions. J. Histochem. Cytochem. 26, 948–959 (1978)
Hardonk, M.J., Hoedemaeker, Ph.J.: Lokalisation der 5′-Nucleotidase-Isoenzyme in der Rattenleber. Acta Histochem. (Jena) Suppl. 10, 385–389 (1969)
Hardonk, M.J., Koudstaal, J.: Enzymehistochemistry as a link between biochemistry and morphology. Prog. Histochem. Cytochem. 8, No. 2 (1976)
Hardonk, M.J., Meskendorp-Haarsma, T.J., Koudstaal, J.: A histochemical study about the influence of lytic enzymes on plasma membrane enzyme. Histochemistry 58, 177–181 (1978)
Hardonk, M.J., Haarsma, T.J., Dijkhuis, F.W.J., Poel, M., Koudstaal, J.: Influence of fixation and buffer treatment on the release of enzymes from the plasma membrane. Histochemistry 54, 57–66 (1977)
Higgins, G.M., Anderson, R.M.: Experimental pathology of liver. I. Restoration of liver of white rats following partial surgical removal. Arch. Pathol. 12, 186–202 (1931)
Koch, H.K., Häberle, B., Meinhardt, K., Enzyn, H., Lesch, R.: Adaptive changes of rat liver cells induced by repeated intraperitoneal injections of D-galactosamine. III. Light- and electron microscopic investigations of hepatocellular cytoplasmic changes. Virchows Arch. B, Cell Pathol. 26, 233–247 (1978)
Longnecker, D.S., Shinozuka, H., Farber, E.: Molecular pathology of in vivo inhibition of protein synthesis. Electron microscopy of rat pancreatic acinar cells in puromycin-induced necroses. Am. J. Pathol. 52, 891–914 (1968)
Longnecker, D.S., Edmonds, T.T.: Electron microscopy of gastric mucosa from puromycin-treated rats. Am. J. Pathol. 57, 65–80 (1969)
Perings, E., Brunner, G., Vido, I., Söling, H.D., Creutzfeld, W.: Protective effects of azathioprine and phenobarbital on acute liver damage produced by galactosamine. Acta Hepato-Gastroenterol. 20, 130–134 (1973)
Reutter, W., Bauer, C., Bachmann, W., Lesch, R.: The galactosamine refractory regenerating rat liver. In: Liver regeneration after experimental injury. R. Lesch and W. Reutter (eds.), pp. 259–272. New York: Stratton Intercontinental Medical Book 1975
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Dedicated to Prof. Dr. E. Havinga on the occasion of his 70th birthday
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Koudstaal, J., Hardonk, M.J. A histochemical study about changes in rat liver plasma membrane enzyme activities after galactosamine administration. Histochemistry 62, 77–84 (1979). https://doi.org/10.1007/BF00537008
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DOI: https://doi.org/10.1007/BF00537008