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Der cytotoxische Einfluß von Plasmin auf Yoshida-Sarkomzellen

The cytotoxic effect of plasmin on Yoshida Sarcoma cells

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Summary

It is possible to determine the death of cells by means of Trypan-blue dye. It could be proved by this method that plasmin exerts a cytotoxic effect on tumor cells. A two hours' exposition to plasmin is sufficient to cause an irreversible damage to tumor cells, i.e. implanting them into rats they do not induce formation of ascites.

Controll experiments using macrophages suggest that tumor cells are especially sensitive to the cytotoxic effect of plasmin. It is supposed that plasmin plays an important role in the body's defensive system towards tumor formation.

Zusammenfassung

Mit Hilfe der Trypanblau-Färbung ist es möglich, den Zelltod in vitro festzustellen. Mit dieser Methode konnte nachgewiesen werden, daß Plasmin einen cytotoxischen Effekt auf Tumorzellen ausübt. Bereits eine zweistündige Plasmineinwirkung ist in der Lage, Tumorzellen irreversibel zu schädigen, so daß diese nach Überimpfung auf Wistarratten keine Ascitesbildung mehr bewirken. Vergleichsuntersuchungen mit Makrophagen sprechen dafür, daß Tumorzellen gegenüber der cytotoxischen Wirkung von Plasmin besonders empfindlich sind. Es wird vermutet daß Plasmin eine wichtige Rolle in der endogenen Tumorabwehr spielt.

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Authors and Affiliations

  1. Abteilung für Hämatologie und Gerinnungsforschung des Zentrums für Innere Medizin der Universität Ulm, Medizinisch-Naturwissenschaftliche Hochschule, Ulm, Deutschland

    V. Hiemeyer, P. Hilgard & R. Kottmann

Authors
  1. V. Hiemeyer
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  2. P. Hilgard
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  3. R. Kottmann
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Additional information

Diese Untersuchungen wurden mit Unterstützung des Badischen Krebsverbandes durchgeführt.

Wesentliche Teile der vorliegenden Arbeit werden von R. Kottmann als Dissertation der Medizinischen Fakultät der Universität Ulm vorgelegt.

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Hiemeyer, V., Hilgard, P. & Kottmann, R. Der cytotoxische Einfluß von Plasmin auf Yoshida-Sarkomzellen. Z Krebs-forsch 72, 36–42 (1969). https://doi.org/10.1007/BF00524790

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  • DOI: https://doi.org/10.1007/BF00524790

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