Summary
Tissue cultures of fibroblasts and carcinoma have been tested radiochemically for the presence of enzymes for the oxidation to carbon dioxide of certain substrates. The tissues were incubated with labelled substrates, and the radioactivity of the CO2 formed measured.
The substrates alanine, glycine, tyrosine, palmitic acid, glucose, fructose and mannose are attacked both by fibroblasts and larynx carcinoma Af. (H. Ep. 2). The oxidation of tyrosine by both kinds of tissue is accelerated by ascorbic acid. Neither tissue oxidises sucrose.
The substrate glycerol is utilised to a higher degree by fibroblasts than by larynx carcinoma. The difference is even more pronounced between fibroblasts and cervix carcinoma HeLa. The implications are discussed.
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Literatur
Weinhouse, S.: Oxidative metabolism of neoplastic tissue. Adv. Cancer Res. 3, 270 (1955).
Olson, R. E.: Oxidation of 14C-labeled carbohydrate intermediates in tumor and normal tissue. Cancer Res. 11, 571 (1951).
Weinhouse, S.: The fate of isotopically labeled metabolites in teh oxidative metabolism of tumors. Cancer Res. 11, 585 (1951).
Weinhouse, S., R. H. Millington and C. E. Wenner: The oxidation of carbohydrates and fatty acids in transplanted tumors. Cancer Res. 11, 845 (1951).
Perschke, H., E. Broda, O. Hoffmann-Ostenhof, L. Stockinger, H. Enzl u. G. Kellner: Anwesenheit bestimmter Enzymsysteme in Gewebekulturen. Mh. Chem. 86, 235 (1956).
Stockinger, L., G. Kellner, E. Broda, H. Perschke and O. Hoffmann-Ostenhof: Über Enzymsysteme des Aminosäureabbaus in Gewebekulturen. Exper. Cell Res. 11, 210 (1956).
Broda, E., u. G. Rohringer: Die Messung von Radiokohlenstoff mit dem Gas-Geiger-Zählrohr. Z. Elektrochem. 58, 634 (1954).
Sverak, L., O. Suschny, G. Manner, E. Broda, R. Stark, L. Stockinger u. H. Enzl: Untersuchung der Atmung einzelner Gewebekulturen mit Radiokohlenstoff. Mh. Chem. 86, 124 (1955).
Kellner, G., L. Stockinger, O. Suschny, E. Broda u. P. Uccusić: Der Einfluß von Cyanid auf einige normale und neoplastische Gewebe. Naturwiss. 43, 472 (1956).
Schönfellinger, H., E. Broda u. L. Stockinger: Papierelektrophoretische Untersuchungen an radioaktiven Gewebekulturen. Mh. Chem. 86, 318 (1955).
Fjelde, A.: Human tumor cells in tissue culture. Cancer Res. 8, 845 (1955).
Fjelde, A.: Observations on human tumour in tissue culture. Nature (Lond.) 175, 434 (1955).
Gey, G. O., W. D. Coffman and M. T. Kubicek: Tissue culture studies of the proliferative capacity of cervical carcinoma and normal epithelium. Cancer Res. 12, 264 (1952).
Scherer, W. F., J. T. Syverton and G. O. Gey: Propagation in vitro of poliomyelitis viruses in cultures of human epithelial cells (strain HeLa) derived from carcinoma of the cervix. J. of Exper. Med. 97, 695 (1953).
Suschny, O., E. Broda, L. Stockinger u. G. Kellner: Atmung und aerobe Glykolyse von Gewebekulturen. In Vorbereitung.
Fulton, W. C., R. Sinclair and I. Leslie: Changes in glucose utilization with the growth of tissue cultures of HeLa cells. Biochemic. J. 63, 18P (1956).
Rous, P., and F. P. Jones: A method for obtaining suspensions of living cells from the fixed tissue for the plating out of individual cells. J. of exper. Med. 23, 546 (1916).
Simms, H. S., and N. P. Stillman: Substances affecting adult tissue in vitro. I. The stimulating action of trypsin an fresh adult tissue. J. Gen. Physiol. 20, 603 (1937).
Weller, T. H., J. F. Enders, F. C. Robins and M. B. Stoddard: Studies on the cultivation of poliomyelitis viruses in tissue culture. I. The propagation of poliomyelitis viruses in suspended cell cultures of various human tissues. J. of Immun. 69, 645 (1952).
Dulbecco, R., and M. Vogt: Plaque formation and isolation of pure lines with poliomyelitis viruses. J. of Exper. Med. 99, 167 (1954).
Youngner, J. S.: Monolayer tissue cultures. I. Preparation and standardization of suspensions of trypsin-dispersed monkey kidney cells. Proc. Soc. Exper. Biol. a. Med. 85, 202 (1954).
Syverton, J. T., and W. F. Scherer: Utilization of a stable strain of human epithelial cells (HeLa, Gey) for diagnosis of poliomyelitis. Federat. Proc. 12, 462 (1953).
Kellner, G., et L. Stockinger: Quantitative Methoden der Gewebezüchtung. Arch. internat. Pharmacodynamie (im Druck).
Doerschuk, A.: The metabolism of glycerol-l-14C. J. of Biol. Chem. 193, 39 (1951).
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Diese Untersuchung wurde durch eine großzügige Subvention des Jane Coffin Childs Memorial Fund for Medical Research unterstützt. Wir danken auch Herrn Prof. H. Lettré, Heidelberg, für die Stämme von Krebsgewebe
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Broda, E., Hoffmann-Ostenhof, O., Perschke, H. et al. Oxydativer Abbau einiger radioaktiver Substrate durch Tumorgewebekulturen. Z Krebs-forsch 61, 504–510 (1957). https://doi.org/10.1007/BF00524626
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DOI: https://doi.org/10.1007/BF00524626