Skip to main content
SpringerLink
Log in

Formation of nitric oxide metabolites during growth of transplanted tumors with different metastatic potential

  • Published:
Biochemistry (Moscow) Supplement Series B: Biomedical Chemistry Aims and scope Submit manuscript

Abstract

The dynamics of the endogenous production of NO metabolites, nitrites, nitrates, and volatile nitrosamines, has been investigated in the body, a tumor tissue, and peritoneal macrophages of mice F1 (C57Bl × CBA), Balb/c and BDF with subcutaneously transplanted tumors: Ehrlich carcinoma (EC) and Lewis metastatic lung carcinoma (LC). It has been shown that during the first three weeks, growth of EC is accompanied by a statistically significant increase in the total concentration of NO metabolites (NOx; nitrites + nitrates) up to levels (7.3 ± 3.49) × 10−6−(7.8 ± 2.57) × 10−5 mol/kg in the tumor tissue and a sharp increase in NOx excretion with urine. At the same time, in the LC tumor tissue the maximal level of NOx (3.6 ± 0.46) × 10−5 mol/kg was registered on day 7, and later it decreased demonstrating negative correlation with the tumor mass. At the stage of intensive LC growth (14, 21 days) there was significant inhibition of nitrite secretion by peritoneal macrophages. In all the controlled time-intervals the EC tumor tissue contained carcinogenic N-nitrosodimethylamine and N-nitrosodiethylamine; however, their concentrations significantly varied. Thus, the ability of the tumor tissue to produce NO metabolites was more pronounced in EC than in LC and depended on the time parameters of tumor growth.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Hibbs, J.B.J., Res. Immunol., 1991, vol. 142, pp. 565–569.

    Article  CAS  Google Scholar 

  2. Granik, V.G. and Grigor’ev, N.B., Oksid azota (Nitric Oxide) Moscow: Vuzovskaya kniga, 2004.

    Google Scholar 

  3. Nathan, C. and Xie, Q.W., J. Biol. Chem., 1994, vol. 269, pp. 13725–13728.

    CAS  Google Scholar 

  4. Bogdan, C., Trends Cell Biol., 2001, vol. 11, pp. 66–75.

    Article  CAS  Google Scholar 

  5. Rockwell, S. and Moulder, J.E., Int. J. Radiat. Oncol. Biol. Phys., 1990, vol. 19, pp. 197–202.

    Article  CAS  Google Scholar 

  6. Melillo, G., Musso, T., Sica, A., Taylor, L.S., Cox, G.W., and Varesio, L., J. Exp. Med., 1995, vol. 182, pp. 1683–1693.

    Article  CAS  Google Scholar 

  7. Pinlaor, S., Spira, B., Ma, N., Hiraku, Y., Yongvanit, P., Wongkham, S., Pairojkul, C., Bhudhisawasdi, V., Oikawa, S., Murata, M., Semba, R., and Kawanishi, S., World J. Gastroenterol., 2005, vol. 11, pp. 4644–4649.

    CAS  Google Scholar 

  8. Wink, D.A., Kasprzak, K.S., Maragos, C.M., Elespuru, R.K., Misra, M., Dunams, T.M., Cebula, T.A., Koch, W.H., Andrews, A.W., and Allen, J.S., Science, 1991, vol. 254, no. 5034, pp. 1001–1003.

    Article  CAS  Google Scholar 

  9. Xu, W., Liu, L.Z., Loizidou, M., Ahmed, M., and Charles, I.G., Cell Research, 2002, vol. 12, pp. 311–320.

    Article  Google Scholar 

  10. Felley-Bosco, E., Cancer Metastasis Rev., 1998, vol. 17, pp. 25–37.

    Article  CAS  Google Scholar 

  11. Jones, L.E., Ying, J.L., Hofseth, A.B., Jelezcova, E., Sobol, R.W., Ambs, S., Harris, C.C., Espey, M.G., Hofseth, L.J., and Wyatt, M.D., Carcinogenesis, 2009, vol. 30, pp. 2123–2129.

    Article  CAS  Google Scholar 

  12. Vakkala, M., Kahlos, K., Lakari, E., Paakko, P., Kinnula, V., and Soini, Y., Clin. Cancer Res., 2000, vol. 6, pp. 2408–2416.

    CAS  Google Scholar 

  13. Garban, H.J. and Bonavida, B., Gynecol. Oncol., 1999, vol. 73, pp. 257–264.

    Article  CAS  Google Scholar 

  14. Nedospasov, A.A., Biochemistry (Moscow), 1998, vol. 63, pp. 881–904.

    Google Scholar 

  15. Bartsch, H., IARC Sci. Publ., 1991, vol. 105, pp. 1–10.

    CAS  Google Scholar 

  16. Thomas, D.D., Ridnour, L.A., Isenberg, J.S., Flores-Santana, W., Switzer, Ch.H., Donzellie, S., Hussain, P., Vecoli, C., Paolocci, N., Ambs, S., Colton, C., Harris, C., Roberts, D.D., and Wink, D.A., Free Rad. Biol. Med., 2008, vol. 45, pp. 18–31.

    Article  CAS  Google Scholar 

  17. Mirvish, S.S., Cancer Lett., 1995, vol. 93, pp. 17–48.

    Article  CAS  Google Scholar 

  18. Miwa, M., Stuehr, D.J., Marletta, M.A., Wishnok, J.S., and Tannenbaum, S.R., IARC Sci. Publ., 1987, vol. 84, pp. 340–344.

    Google Scholar 

  19. Deryagina, V.P. and Ryzhova, N.I., Vestnik Blokhin RONC RAMN, 2007, vol. 18, pp. 32–37.

    Google Scholar 

  20. Choudhari, Sh.K., Chaudhary, M., Bagde, S., Gadbail, A.R., and Joshi, V., World J. Oncol., 2013, vol. 11, p. 118.

    Article  Google Scholar 

  21. Preussmann, R., Naturwissenschaften, 1984, vol. 71, pp. 25–30.

    Article  CAS  Google Scholar 

  22. Thomas, D.D., Espey, M.G., Ridnour, L.A., Hofseth, L.J., Mancardi, D., Harris, C.C., and Wink, D.A., Proc. Natl. Acad. Sci. USA, 2004, vol. 101, pp. 8894–8899.

    Article  CAS  Google Scholar 

  23. Tsikas, D., J. Chromatogr., 2007, vol. 851, pp. 51–70.

    CAS  Google Scholar 

  24. Opredelenie letuchikh N-nitrozoaminov v prodovol’stvennom syr’e i pishchevykh produktakh. Metodicheskie ukazaniya po metodam kontrolya (Determination of Volatile N-Nitrosoamines in Food Products. Methodical Recommendations on the Controlling Methods), 1993, MUK 4.4.1.011-93.

  25. Ridnour, L.A., Thomas, D.D., Mancardi, D., Espey, M.G., Miranda, K.M., Paolocci, N., Feelisch, M., Fukuto, J., and Wink, D.A., Biol. Chem., 2004, vol. 385, pp. 1–10.

    Article  CAS  Google Scholar 

  26. Cleeter, M.W., Cooper, J.M., Darley-Usmar, V.M., Moncada, S., and Schapira, A.H., FEBS Lett., 1994, vol. 345, pp. 50–54.

    Article  CAS  Google Scholar 

  27. Vanin, A.F., Mordvintcev, P.I., Hauschildt, S., and Mulsch, A., Biochim. Biophys. Acta, 1993, vol. 1177, pp. 37–42.

    Article  CAS  Google Scholar 

  28. Gaston, B., Reilly, J., Drazen, J.M., Fackler, J., Ramdev, P., Arnelle, D., Mullins, M.E., Sugarbaker, D.J., Chee, C., and Singel, D.J., Proc. Natl. Acad. Sci. USA 1993, vol., 90, pp. 10957–10961.

    Article  CAS  Google Scholar 

  29. Osipov, A.N., Borisenko, G.G., and Vladimirov, Yu.A., Usp. Biol. Khim., 2007, vol. 47, pp. 259–292.

    CAS  Google Scholar 

  30. Reutov, V.P., Sorokina, E.G., Okhotin, V.E., and Kositsyn, N.S., Tsiklicheskie prevrashcheniya oksida azota v organizme mlekopitayushchikh (Cyclic Conversion of Nitric Oxide in Mammalian Organisms), Moscow: Nauka, 1997.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Carcinogenesis, Blokhin Russian Cancer Research Center, Kashirskoye sh. 24, Moscow, 115478, Russia

    V. P. Deryagina, N. I. Ryzhova & L. V. Krivosheeva

  2. Institute of Experimental Diagnostics and Therapy of Cancer, Blokhin Russian Cancer Research Center, Moscow, Russia

    I. S. Golubeva

Authors
  1. V. P. Deryagina
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. N. I. Ryzhova
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. L. V. Krivosheeva
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. I. S. Golubeva
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to V. P. Deryagina.

Additional information

Original Russian Text © V.P. Deryagina, N.I. Ryzhova, L.V. Krivosheeva, I.S. Golubeva, 2015, published in Biomeditsinskaya Khimiya.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Deryagina, V.P., Ryzhova, N.I., Krivosheeva, L.V. et al. Formation of nitric oxide metabolites during growth of transplanted tumors with different metastatic potential. Biochem. Moscow Suppl. Ser. B 9, 130–136 (2015). https://doi.org/10.1134/S1990750815020055

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S1990750815020055

Keywords

Search

Navigation