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The HLA-D system: At least two loci and four distinct phenotypic traits per haplotype. Introduction to component typing in families and population by primed lymphocyte typing

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Abstract

Using a number of intrafamilial PLTs raised against identical HLA haplotypes it has been possible to construct a model in an informative family defining the HLA-D region as a genetic system. This system consists of at least two regions separated by a recombination between HLA-D and GLO. In relation to the site of recombination, a minimum of one centromeric and three telomeric components can be identified per haplotype.—Fourteen PLTs raised and defined within the family were subsequently tested in a Caucasian population (n=84) and in 13 unrelated, complete families.—It is concluded that the hypothetical model proposed for the HLA-D region as a genetic system of linked loci, coding at the cell surface for associated but distinct components (at least four per haplotype), allows for typing of the components of the HLA-D system of any given haplotype. Serological typing of HLA-D components should, in the near future, provide a more convenient way of establishing component phenotypes than the present use of primed lymphocyte typing reagents. Among the components isolated, some have a high association with the classic alleles defined either by homozygous typing cells or DR serology. Others form the basis of cross-reactivity but their presence does not interfere with standard typing. Others, however, seem by their mere presence to be responsible for false assignments.—The concept of HLA-D as a genetic system clarifies many of the inconsistencies observed with a one-locus system.

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  1. Centre d'Immunologie INSERM-CNRS de Marseille-Luminy, Case 906, 13288, Marseille Cedex 9, France

    Dominique Charmot, Claude Mawas, Tom Kristensen & Pierre Mercier

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  1. Dominique Charmot
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  2. Claude Mawas
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  3. Tom Kristensen
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  4. Pierre Mercier
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Research scientists from INSERM.

Research Fellow from the Danish Medical Research Council.

Central Blood Bank — Marseille

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Charmot, D., Mawas, C., Kristensen, T. et al. The HLA-D system: At least two loci and four distinct phenotypic traits per haplotype. Introduction to component typing in families and population by primed lymphocyte typing. Immunogenetics 13, 57–84 (1981). https://doi.org/10.1007/BF00524605

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