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RNS-Synthese der Haut

DNS-abhängige RNS-Polymerase-Aktivität in Meerschweinchen-Epidermis

RNA-synthesis of the skin

DNA-dependent RNA-polymerase activity in guinea pig epidermis

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Summary

  1. 1.

    The activity of DNA-dependent RNA-polymerase has been determined in isolated epidermal cells using an in vitro RNA synthesizing system.

  2. 2.

    The DNA-dependent RNA-polymerasereaction shows characteristics which are identical to those described in other organs for this enzyme. The reaction reaches a plateau after 16–20 min and is inhibited by Actinomycin and DNase. The product is hydrolysed by RNase. The reaction is dependent on the presence of 4 nucleosid-triphosphates and divalent cations in the in-vitro-system.

  3. 3.

    Addition of ammoniumsulfate in high concentrations increases the polymerase activity several times.

Zusammenfassung

  1. 1.

    Die Aktivität der DNS-abhängigen RNS-Polymerase in isolierten Epidermiszellen wurde in einem in vitro RNS-synthetisierenden System gemessen und die Enzymreaktion charakterisiert.

  2. 2.

    Die DNS-abhängige RNS-Polymerasereaktion zeigt die gleichen Charakteristica wie sie für dieses Enzym in anderen Organen beschrieben wurden. Die Reaktion erreicht ihr Plateau nach 16–20 min und wird durch Actinomycin und DNase gehemmt. RNase hydrolysiert das Produkt. Die Reaktion ist abhängig von der Anwesenheit der 4 Nucleosidtriphosphate und divalenten Kationen in dem in vitro-System.

  3. 3.

    Der Zusatz von Ammoniumsulfat in hohen Konzentrationen zu der Inkubationsmischung erhöht die Polymeraseaktivität auf das Vielfache.

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Authors and Affiliations

  1. Dermatologische Klinik und Poliklinik der Ludwig Maximilians-Universität München, München, Deutschland

    I. Lukács & O. Braun-Falco

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  1. I. Lukács
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  2. O. Braun-Falco
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Die Untersuchungen wurden mit Unterstützung der Deutschen Forschungsgemeinschaft durchgeführt.

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Lukács, I., Braun-Falco, O. RNS-Synthese der Haut. Arch. klin. exp. Derm. 238, 187–196 (1970). https://doi.org/10.1007/BF00519904

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  • DOI: https://doi.org/10.1007/BF00519904

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