Summary
An isolation procedure for sarcolemma of human skeletal muscle is described. The method includes the possibility to prepare sarcoplasmic reticulum from the same muscle fibres. Electron microscopy reveals a homogeneous final fraction of 80–90% myofibre enveloping membranes contaminated by blood vessel membranes. The typical three-laminar composition of isolated sarcolemma is demonstrated. The mechanism of muscle fibre emptying is discussed.
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Herrn Professor Dr. Werner Scheid zur Vollendung seines 65. Lebensjahres gewidmet.
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Engel, H., Gorgas, K. Isolation of sarcolemma from human skeletal muscle. Z. Anat. Entwickl. Gesch. 145, 187–196 (1974). https://doi.org/10.1007/BF00519728
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DOI: https://doi.org/10.1007/BF00519728