Abstract
Ribosomes were obtained from spores of Aspergillus fumigatus by mechanical disruption and differential centrifugation. The initial preparation (crude ribosomes) contained spore components which appeared to be broken fragments of plasmalemma with or without organelles. Purified ribosomes free of membranous material were prepared by gel filtration chromatography on Sepharose CL-4B. Monomeric 80S ribosomes consisting of 40% protein and 60% RNA, and morphologically characteristic of fungal ribosomes were isolated.
Serological reactions in sera from rabbits injected with crude or purified ribosomes were similar indicating that the purification process did not change or eliminate antigens that stimulated antibody detectable either by indirect hemagglutination (IHA) or in gel precipitin tests.
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Almog, R. & Shirey, T. L., 1978. A modified orcinol test for the specific determination of RNA. Anal. Biochem. 91: 130–137.
Crawford, R. P., 1972. Molecular characteristics of antibody detected by MA test in serum of guinea pigs with leptospirosis. Am. J. Vet. Res. 33: 1507–1512.
Eisenstein, T. K., 1975. Evidence for 0 antigens as the antigenic determinants in ‘ribosomal’ vaccines prepared from Salmonella. Infec. Immun. 12: 364–377.
Eubanks, E. R., Leathers, C. R., Sanchez, A., Todd, C. D. & Katz, G.T., 1979. Evaluation of subcellular fractions of Coccidioides immitis as protective immunogens. Abstract of Am. Soc. Microbiol. p. 372.
Feit, C. & Tewari, R. P., 1974. Immunogenicity of ribosomal preparations from yeast cells of Histoplasma capsulatum. Infec. Immun. 10: 1091–1097.
Garvey, J. S., Cremer, H. E. & Sussdorf, D. H., 1977. Methods in immunology. 3rd ed., W. A. Benjamin, Inc., Reading MA, p. 522.
Hawker, L. E. & Madelin, M. F., 1976. The dormant spore. In The Fungal Spore, D. J. Weber & W. M. Hess, Eds. pp. 7–72, John Wiley & Sons, NY.
Misfeldt, M. L. & Johnson, W., 1978. Identification of protective cell surface proteins in ribosomal fractions from Salmonella typhimurium. Infec. Immun. 24: 808–816.
Nieuwenhuysen, P., DeHerdt, E. & Clauwaert, J., 1978. Ribonucleic acid and protein content of eukaryotic ribosomes isolated from Artemia salina. Anal. Biochem. 88: 532–538.
Raper, K. B. & Fennel, D. I., 1965. The genus Aspergillus. p. 686. Williams & Wilkins Co., Baltimore, MD.
Rheins, M. S., Thurston, J. R., Newton, N. & Dodd, M. C., 1954. Evaluation of ‘drop’ techniques for the detection of hemagglutinins and hemolysins in tuberculous sera. Am. J. Med. Technol. 20: 254–258.
Richard, J. L., Cysewski, S. J. & Fichtner, R. E., 1971. Harvest and survival of Aspergillus fumígatus fresnius spores. Mycopathol. Mycol. Appl. 43: 165–168.
Richard, J. L., Thurston, J. R., Cutlip, R. C. & Pier, A. C., 1981. Vaccine studies of Aspergillosis in Turkey I subcutaneous inoculation with several vaccine preparations followed by aerosol challenge exposure. In press. Am. J. Vet. Res.
Saunders, E. S., Solotorovsky, M. & Tewari, R. P., 1977. Characterization of the immunogenic moiety associated with ribosomes of Candida albicans. Abstracts of Am. Soc. Microbiol. p. 123.
Segal, E., Levy, R. & Eylan, E., 1978. Antibody formation in experimental immunizations with Candida albicans ribosomal fractions. Mycopathologia 64: 121–123.
Spirin, A. S., 1972. Structure of the ribosome. In Progress in Biophysics and Molecular Biology, J. A. V. Butler and D. Noble, Eds. pp. 133–173. Pergamon Press, New York.
Taylor, M. M. & Storck, R., 1964. Uniqueness of bacterial ribosomes. Proc. Natl. Acad. Science 52: 958–965.
Thurston, J. R., Cysewski, S. J., Pier, A. C. & Richard, J. L., 1972. Precipitins in serums from sheep infected with Aspergillus fumigatus. Am. J. Vet. Res. 33: 929–933.
Thurston, J. R., Richard, J. L., Cysewski, S. J. & Fichtner, R. E., 1975. Antibody formation in rabbits exposed to aerosols containing spores of Aspergillus fumigatus. Am. J. Vet. Res. 36: 899–901.
Weisberg, S.H. & Turian, 1971. Ultrastructure of Aspergillus nidulans conidia and conidial lomasomes. Protoplasma 72: 55–67.
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Phillips, M., Thurston, J.R., Brogden, K.A. et al. Isolation of 80S ribosomes from spores of Aspergillus fumigatus and their antigenicity in rabbits. Mycopathologia 77, 165–171 (1982). https://doi.org/10.1007/BF00518802
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DOI: https://doi.org/10.1007/BF00518802