Abstract
Oxygen and shear stress are the key factors for enhanced glucan production with Schizophyllum commune. During batch cultivation control of \(P_{O_2 } \) or \(q_{O_2 } \) (specific oxygen uptake rate) was achieved by variation of the impeller speed. Biomass was modelled by using the carbon and oxygen balance derived from exhaust data. At mycel growth a \(q_{O_2 } \) of 0.042 h−1 presents just the border before oxygen limitation arises and is simultaneously the optimum operation condition for maximum glucan formation. Related to an overall cultivation time of 72 h a maximum of both productivity (4.3 kg m−3 d−1) and yield (13 kg m−3) were obtained.
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Abbreviations
- C kg m−3 :
-
concentration
- k L a h −1 :
-
volume related oxygen transfer coefficient
- K s mol m−3 :
-
substrate saturation constant
- N rpm:
-
impeller speed
- \(P_{O_2 } \) %:
-
oxygen partial pressure of the liquid phase
- \(Q_{O_2 } \) kg m−3h−1 :
-
oxygen uptake rate
- \(q_{O_2 } \) h−1 :
-
specific oxygen uptake rate, kg O2 (kg biomass h)−1
- t h:
-
time
- \(Y_{X/O_2 } \) :
-
yield coefficient (biomass formed/oxygen consumed)
- μ h−1 :
-
specific growth rate
- O 2 :
-
oxygen
- X :
-
biomass
- L :
-
liquid phase
- *:
-
gas/liquid interface
- S :
-
substrate (glucose)
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Dedicated to the 65th birthday of Professor Fritz Wagner.
This work was kindly supported in parts by B. Braun Biotech International. The authors are grateful to Prof. Dr. Fritz Wagner for scientific support and appreciate the technical assistance of Detlev Rasch
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Rau, U., Brandt, C. Oxygen controlled batch cultivations of Schizophyllum commune for enhanced production of branched β-1,3-glucans. Bioprocess Engineering 11, 161–165 (1994). https://doi.org/10.1007/BF00518738
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DOI: https://doi.org/10.1007/BF00518738