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A perfusion method of incubation to demonstrate horseradish peroxidase in bone

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Summary

A perfusion method of incubation to show horseradish peroxidase in the bone of young mice is presented. After perfusion fixation, the incubation medium is perfused from the descending aorta into the entire lower half of the animal. From the vessels there is good penetration of the medium into all tissues. This allows the preparation of any one perfused bone to ground-, semithin-, and ultrathin sections.

Differences in peroxidase distribution in the entire bone suggest regional differences in vascular supply.

The tracer enzyme diffuses freely from the vessels into the extracellular fluid of bone. 3 min after injection, peroxidase is found between all bone lining cells and in osteocyte lacunae.

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References

  • Böck, P.: Das Glomus caroticum der Maus. Adv. Anat. Embryo. Cell Biol. 48, 1–84 (1973)

    Google Scholar 

  • Bussolati, G.: Histochemical demonstration of sulphate groups by means of diaminobenzidine, Histochemical J. 3, 445–449 (1971)

    Google Scholar 

  • Glauman, H.: Demonstration of Phosphatases by Perfusion incubation, Histochemistry 44, 169–178 (1975)

    Google Scholar 

  • Karnovsky, M.J.: The ultrastructural basis of capillary permeability studied with peroxidase as a tracer, J. Cell Biol. 35, 213–236 (1967)

    Google Scholar 

  • Lucht, U.: Absorption of Peroxidase by Osteoclasts as Studied by Electron Microscope. Histochemie 29, 274–286 (1972)

    Google Scholar 

  • Luft, J.H.: Improvements in epoxy resin embedding methods, J. biophys. biochem. Cytol. 9, 409–414 (1961)

    Google Scholar 

  • Neuman, W.F., Mulryen, B.J., Neuman, M.W., Lane, K.: Calcium transport systems in the chick calvaria. Amer. J. Physiol. 224, 600–606 (1973)

    Google Scholar 

  • Palade, G.E.: A study of fixation for Electron microscopy. J. exp. Med. 95, 285–298 (1952)

    Google Scholar 

  • Scarpace, P.J., Neuman, W.F.: The Blood: Bone Disequilibrium I. Calcif. Tissue Res. 20, 137–149 (1976)

    Google Scholar 

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Authors and Affiliations

  1. Laboratory for Bone Research, Institute of Histology and Embryology, University of Vienna, Schwarzspanierstraße 17, A-1090, Vienna, Austria

    Markus Lorenz & Hanns Plenk Jr.

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  1. Markus Lorenz
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  2. Hanns Plenk Jr.
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Lorenz, M., Plenk, H. A perfusion method of incubation to demonstrate horseradish peroxidase in bone. Histochemistry 53, 257–263 (1977). https://doi.org/10.1007/BF00511081

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  • DOI: https://doi.org/10.1007/BF00511081

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