Summary
Dopamine-containing vesicles were isolated from the caudate nucleus of the pig by differential centrifugation and incubated with 14C-serotonin (14C-5-HT), 14C-gamma-aminobutyric acid (14C-GABA), 14C-dopamine or 14C-histamine. At 25°C the uptake of 14C-5-HT and 14C-GABA was enhanced by ATP and magnesium. The Km for the uptake of 14C-5-HT in the presence of ATP and magnesium was 0.67×10−6 M, that of 14C-GABA 1.33×10−4 M. 14C-Histamine was also taken up into the vesicles but its uptake was not influenced by ATP and magnesim. The ATP-magnesium-dependent uptake of 14C-5-HT and 14C-GABA was abolished at 0°C. At 37°C the accumulation of 14C-GABA in the presence of ATP and magnesium reached a steady state after 20 min, while the accumulation of 14C-5-HT reached a maximum after 2.5 min of incubation and then gradually declined. Osmotic lysis of the vesicles followed by sonication abolished the enhanced uptake of 14C-GABA, 14C-5-HT and 14C-dopamine in the presence of ATP and magnesium. Determination of the endogenous contents of the vesicles revealed 14.6 pmoles per mg protein for 5-HT and 22.7 nmoles per mg protein for GABA. It is concluded that the caudate nucles preparation used in this study contains not only dopamine-storing vesicles able to take up biogenic amines but also GABA-containing vesicles which take up GABA by an ATP-magnesium-dependent process.
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This work was supported by the Deutsche Forschungsgemeinschaft and the Stiftung Volkswagenwerk.
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Philippu, A., Matthaei, H. Uptake of serotonin, gamma-aminobutyric acid and histamine into synaptic vesicles of the pig caudate nucleus. Naunyn-Schmiedeberg's Arch. Pharmacol. 287, 191–204 (1975). https://doi.org/10.1007/BF00510450
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DOI: https://doi.org/10.1007/BF00510450