Summary
Adrenaline and noradrenaline produced both constriction and dilatation of the toad renal vasculature: constrictor effects were mediated by α-adrenoceptors; dilator effects were mediated by β-adrenoceptors. Vasoconstriction was the predominant response to these amines. Dilatation was only revealed after blockade of α-adrenoceptors and constriction of the vasculature. There was a marked seasonal variation in the constrictor responses to adrenaline, but not to noradrenaline. The maximal increase in renal vascular resistance produced by adrenaline in summer was greater, by a factor of three, than the maximum constrictor response in winter. The response to adrenaline in summer was also significantly greater than the responses to noradrenaline in both summer and winter. However, after treatment with propranolol there was no difference between the maximum vasoconstrictions to these two amines, in summer or in winter. Determination of dissociation constants for phentolamine indicated that adrenaline and noradrenaline acted on the same population of α-adrenoceptors in both summer and winter. The enhanced vasoconstriction to adrenaline in summer appears to be due to a reduced potency of adrenaline on renal vascular β-adrenoceptors. The reduction in potency may be caused by a subtle configurational change in the β-adrenoceptors, perhaps induced by hormonal changes associated with the onset of breeding.
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References
Altura BM (1978) Pharmacology of venular smooth muscle: new insights. Microvasc Res 16:91–117
Ariens EJ, Simonis AM (1961) Analysis of the action of drugs and drug combinations. In: Jonge H (ed) Quantitative methods in pharmacology. North Holland, Amsterdam, pp 286–311
Atkinson JM, Rand MJ (1972) Reduction of cardiovascular response to some sympathomimetic amines during hypercapnia. Eur J Pharmacol 18:166–173
Barcroft J, Izquierdo JJ (1931) The relation of temperature to the pulse rate of the frog. J Physiol 71:145–155
Baron GD, Speden RN, Bohr DF (1972) Beta-adrenergic receptors in coronary and skeletal muscular arteries. Am J Physiol 223:878–881
Benfey BG (1979) Cardiac adrenoceptors at low temperatures and the adrenoceptor interconversion hypothesis. Can J Physiol Pharmacol 57:771–777
Bevan JA (1979) Some bases of differences in vascular response to sympathetic activity. Variation on a theme. Circ Res 45:161–171
Boyd IA, Pathak CL (1965) The response of perfused frog hearts to minute quantities of acetylcholine, and the variation in sensitivity with season. J Physiol 176:191–204
Buckley GA, Jordan CC (1970) Temperature modulation of α- and β-adrenoceptors in the isolated frog heart. Br J Pharmacol 38: 394–398
Cohen ML, Wiley KS (1977) Specific enhancement of norepinephrineinduced contraction in rat veins after beta-adrenergic antagonists. J Pharmacol Exp Ther 201:406–416
Donosa AO, Segura ET (1965) Seasonal variations of plasma adrenaline and noradrenaline in toads. Gen Comp Endocrinol 5:440–443
Fleisch JH, Maling HM, Brodie BB (1970) Beta-receptor activity in aorta. Variations with age and species. Circ Res 26:151–162
Hyman AL, Nandiwada P, Knight DS, Kadowitz PJ (1981) Pulmonary vasodilator responses to catecholamines and sympathetic nerve stimulation in the cat. Evidence that vascular β2-adrenoceptors are innervated. Circ Res 48:407–415
Kleinstein J, Glossmann H (1978) Solubilisation of a mammalian β-adrenergic receptor. Naunyn-Schmiedeberg's Arch Pharmacol 305: 219–225
Kunos G, Szentiványi M (1968) Evidence favouring the existence of a single adrenergic receptor. Nature 217:1077–1078
Kunos G, Nickerson M (1976) Temperature-induced interconversion of α- and β-adrenoceptors in the frog heart. J Physiol 256:23–40
Kunos G, Mucci L, O'Regan S (1980) The influence of hormonal and neuronal factors on rat heart adrenoceptors. Br J Pharmacol 71: 371–386
Lande IS de la, Parker DAS, Morris R, Irvine R, Graefe KH (1980) Noradrenaline diffusion across the artery. In: Bevan JA, Godfraid T, Maxwell RA, Vanhoutte PM (eds) Vascular neuroeffector mechanisms. Raven Press, New York, pp 148–160
Levin RM, Shofer FS, Wein AJ (1980) Estrogen-induced alterations in the autonomic responses of the rabbit urinary bladder. J Pharmacol Exp Ther 215:614–618
Loh D von (1971) The effect of adrenergic drugs on spontaneously active vascular smooth muscle studied by long-term intracellular recording of membrane potential. Angiologica 8:144–155
Lund GF, Dingle H (1968) Seasonal temperature influence on vagal control of diving bradycardia in the frog (Rana pipiens). J Exp Biol 48:265–277
MacLeod KM, McNeill JH (1981) The influence of altered thyroid hormone levels on guinea-pig cardiac adrenoceptors. Can J Physiol Pharmacol 59:1039–1049
Morris JL, Campbell G (1978) Renal vascular anatomy of the toad (Bufo marinus). Cell Tissue Res 189:501–514
Paiva MW, Guimarâes S (1978) A comparative study of the uptake and metabolism of noradrenaline and adrenaline by the isolated saphenous vein of the dog. Naunyn-Schmiedeberg's Arch Pharmacol 303:221–228
Pegram BL, Bevan RD, Bevan JA (1976) Facial vein of the rabbit: neurogenic vasodilatation mediated by beta-adrenergic receptors. Circ Res 39:854–860
Segura ET, D'Agostino SA (1964) Seasonal variations of blood pressure, vasomotor reactivity, and plasmatic catecholamines in the toad. Acta Physiol Lat Am 14:231–237
Segura ET, D'Agostino SA (1965) Sexual factors affecting blood pressure and vasomotor reactivity in toads. Gen Comp Endocrinol 5: 278–283
Shepherd JT, Vanhoutte PM (1975) Veins and their control. Saunders, Philadelphia
Sokal RR, Rohlf FJ (1969) Biometry. The principles and practice of statistics in biological research. Freeman, San Francisco
Stene-Larsen G, Helle KB (1979) Temperature effects on the inotropic and chronotropic responses to adrenaline in the frog heart. J Comp Physiol 132:313–318
Stier TJB, Bock HC (1966) Seasonal changes of heart rate-temperature relationships in toads. Proc Soc Exp Biol Med 123:149–151
Stone M, Angus JA (1978) Developments of computer-based estimation of pA2 values and associated analysis. J Pharmacol Exp Ther 207:705–718
Thomas JA, Sakai KK, Holck MI, Marks BH (1980) Dopamine-betahydroxylase: a modulator of beta-adrenergic receptor activity. Res Commun Chem Path Pharmacol 29:3–13
Vanhoutte PM (1978) Heterogeneity in vascular smooth muscle. In: Kaley G, Altura BM (eds) Microcirculation, vol 2. University Park Press, Baltimore, pp 181–309
Vanhoutte PM, Shepherd JJ (1970) Effect of cooling on beta-receptor mechanisms in isolated cutaneous vein of the dog. Microvasc Res 2:454–461
Voelkel NF, Hegstrand L, Reeves JT, McMurty IF, Molinoff PB (1981) Effects of hypoxia on density of β-adrenergic receptors. J Appl Physiol 50:363–366
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Morris, J.L. Seasonal variation in responses of the toad renal vasculature to adrenaline. Naunyn-Schmiedeberg's Arch. Pharmacol. 320, 246–254 (1982). https://doi.org/10.1007/BF00510136
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DOI: https://doi.org/10.1007/BF00510136