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Aryl hydrocarbon hydroxylase activity in cultured lymphocytes of psoriatic patients

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Summary

To evaluate further the metabolic capacity of psoriatics, the activities and inducibility of aryl hydrocarbon hydroxylase (AHH) were measured in cultured mitogen-stimulated lymphocytes from the peripheral blood of 68 psoriatic and 39 control patients. There was no significant difference in the mean basal and induced AHH activities between the psoriatics and the controls, although the former showed a slight tendency towards a higher inducibility ratio. In both, the basal and induced AHH values decreased significantly with increasing age. The cigarette smokers in both groups had significantly higher AHH activities than the nonsmokers. Antipsoriatic treatement (Anthralin, PUVA) did not seem to influence the lymphocyte AHH activities. No association was demonstrable between the AHH activities and the duration of the disease.

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References

  1. Alvares AP, Kappas A, Lewin W, Conney AH (1973) Inducibility of benzo(a)pyrene in human skin by polycyclic hydrocarbons. Clin Pharmacol Ther 14:30–39

    Google Scholar 

  2. Atlas SA, Vesell ES, Nebert DW (1976) Genetic control of interindividual variation in the inducibility of aryl hydrocarbon hydroxylase in cultured human lymphocytes. Cancer Res 36:4919–4930

    Google Scholar 

  3. Bickers DR, Kappas A (1978) Human skin aryl hydrocarbon hydroxylase, induction by coal tar. J Clin Invest 62:1061–1068

    Google Scholar 

  4. Böyum A (1968) Separation of leucocytes from blood and bone marrow with special references to factors which influence and modify sedimentation properties of hematopoietic cells. Scand J Clin Lab Invest 21 [Suppl] 97:1–109

    Google Scholar 

  5. Chapman PH, Kersey PJ, Keys B, Shuster S, Rawlins MD (1980) Generalized tissue abnormality of aryl hydrocarbon hydroxylase in psoriasis. Br Med J 281:1315–1316

    Google Scholar 

  6. Chapman PH, Keys B, Rawlins MD, Moss C, Shuster S (1981) Antipyrine clearance in psoriasis. Br Med J 282:20–21

    Google Scholar 

  7. Gahmberg CG, Sekki A, Kousen TV, Holsti LR, Mäkelä O (1979) Induction of aryl hydrocarbon hydroxylase activity and pulmonary carcinoma. Int J Cancer 23:302–305

    Google Scholar 

  8. Gelboin HV (1980) Venzo(a)pyrene metabolism, activation, and carcinogenesis: Role and regulation of mixed-function oxidase and related enzymes. Physiol Rev 60:1107–1166

    Google Scholar 

  9. Gillette JR (1979) Biotransformation of drugs during aging. Fed Proc 38:1900–1909

    Google Scholar 

  10. Gurtoo HL, Bejba N, Minowada J (1975) Properties, inducibility, and an improved method of analysis of aryl hydrocarbon hydroxylase in cultured human lymphocytes. Cancer Res 35:1235–1243

    Google Scholar 

  11. Hannuksela M, Karvonen J (1978) Trioxalen bath plus UVA effective and safe in the treatment of psoriasis. Br J Dermatol 9:703–707

    Google Scholar 

  12. Jusko WJ, Schentag JJ, Clark JH, Gardner M, Yurchak AM (1978) Enhanced biotransformation of theofylline in marihuana and tobacco smokers Clin Pharmacol Ther 24:406–410

    Google Scholar 

  13. Kärki NT, Huhti E (1978) Aryl hydrocarbon hydroxylase activity in cultured lymphocytes from lung carcinoma patients and cigarette smokers. Abstr 7th Int Congr Pharmacol, No 644, p 254

  14. Kärki NT, Pelkonen O, Tuimala R, Kaupplia A, Koivisto M (1981) Aryl hydrocarbon hydroxylase induction in maternal and cord blood mitogen treated lymphocytes. Dev Pharmacol Ther 2:32–43

    Google Scholar 

  15. McLemore TL, Russell RM, Busbee DL, Richie RC, Springer PR, Toppell KE, Cantrell ET (1977) Aryl hydrocarbon hydroxylase activity in pulmonary macrophages and lymphocytes from lung cancer and noncancer patient. Cancer Res 37:1175–1181

    Google Scholar 

  16. Mustakallio KK (1979) Trritation and staining by dithranol (anthralin) and related compounds. I. Estimation with chamber testing and contact thermography. Acta Derm Venereol (Stockh) [Suppl 85] 59:125–132

    Google Scholar 

  17. Nebert DW, Gelboin HV (1968) Substrate-induced microsomal aryl hydroxylase in mammalian cell culture. I. Assay and properties of induced enzyme. J Biol Chem 243:6242–6249

    Google Scholar 

  18. O'Malley K, Crooks J, Duke E, Stevenson IH (1971) Effect of age and sex on human drug metabolism. Br Med J 3:607–609

    Google Scholar 

  19. Pelkonen O (1976) Metabolism of benzo(a)pyrene in human adult and fetal tissues. In: Freudenthal RI, Jones PW (eds) Carcinogenesis, vol 1. Polynuclear aromatic hydrocarbons: Chemistry, metabolism and carcinogenesis. Raven Press, New York, pp 9–21

    Google Scholar 

  20. Ruzicka T, Vizethum W, Jacobs A, Goerz G (1980) Aryl hydrocarbon hydroxylase activity in lymphocytes of patients with psoriasis. Lancet 1:1142–1143

    Google Scholar 

  21. Shuster S, CHapman P, Rawlins MD (1979) Psoriasis and cancer. Br Med J 1:941–942

    Google Scholar 

  22. Shuster S, Rawlins MD, Chapman PH, Rogers S (1980) Decreased epidermal aryl hydrocarbon hydroxylase and localized pustural psoriasis. Br J Dermatol 103:23–26

    Google Scholar 

  23. Stern SS, Zierler S, Parrish JA (1980) Skin carcinoma in patients with psoriasis treated with topical tar and artificial ultraviolet radiation. Lancet 1:732–735

    Google Scholar 

  24. Thorgeirsson SS, Nebert DW (1977) The Ah Locus and the metabolism of chemical carcinogens and other foreign compounds. Adv Cancer Res 2:149–193

    Google Scholar 

  25. Vesell ES, Page JG (1968) Genetic control of drug level in man. J Clin Invest 47:2657–2663

    Google Scholar 

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Authors and Affiliations

  1. Department of Pharmacology, University of Oulu, Kajaanintie 52 D, SF-90220, Oulu 22, Finland

    Niilo T. Kärki, J. Karvonen & O. Pelkonen

  2. Department of Dermatology, University of Oulu, Kajaanintie 52 D, SF-90220, Oulu 22, Finland

    Niilo T. Kärki, J. Karvonen & O. Pelkonen

Authors
  1. Niilo T. Kärki
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  2. J. Karvonen
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  3. O. Pelkonen
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Kärki, N.T., Karvonen, J. & Pelkonen, O. Aryl hydrocarbon hydroxylase activity in cultured lymphocytes of psoriatic patients. Arch Dermatol Res 273, 103–109 (1982). https://doi.org/10.1007/BF00509033

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  • DOI: https://doi.org/10.1007/BF00509033

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