Summary
Lithocholic acid 24-C14 is converted by 20000x g-supernatant of rat liver homogenate into several products of higher polarity. 3α-6β-dihydroxy-5β-cholanic acid is the main metabolite, whereas 7α-hydroxylation occurs only to a small extent. Besides of the hydroxylations conjugation with taurine and the formation of a 3-sulfate ester of lithocholic acid can be demonstrated. Addition of ethanol to the enzymatic system results in an inhibition of the formation of 3α,6β-dihydroxy-5β-cholanic acid, whereas no effect can be observed with respect to the formation of the other products. This inhibition is present also in 20000x g-supernatant obtained from rats pretreated with ethanol 100 min before being preparation from ethanol-pretreated rats amounts to 68% of the controls. The chrome P-450 mediated oxidative mechanism, which has been shown to be involved in microsomal 6β-hydroxylation of bile acids.
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This work was supported by the Deutsche Forschungsgemeinschaft.
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Back, P., Schernau-Potzi, L. Effect of ethanol on the metabolism of lithocholic acid in rat liver. Naunyn-Schmiedeberg's Arch. Pharmacol. 275, 135–145 (1972). https://doi.org/10.1007/BF00508902
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DOI: https://doi.org/10.1007/BF00508902