Naunyn-Schmiedeberg's Archives of Pharmacology

, Volume 286, Issue 4, pp 389–400 | Cite as

In vivo rates of tyrosine and tryptophan hydroxylation in regions of rat brain at four times during the light-dark cycle

  • Jean DiRaddo
  • Carol Kellogg


The in vivo rates of tyrosine and tryptophan hydroxylation were studied in three regions of young adult rat brains at 4 times during the light-dark cycle. The procedure utilized was to analyze the accumulation of Dopa and 5-HTP after injection of a centrally effective L-amino acid decarboxylase inhibitor, NSD 1015. Monoamine levels were also determined in all control animals and some treated animals. The rate of tyrosine hydroxylation in the telencephalon was significantly higher 7 hrs after dark onset than at the other three times tested. Smaller variations in tyrosine and tryptophan hydroxylation rates as a function of time of day were also observed. 5-HT levels were significantly higher during the light phase than the dark in the telencephalon with the same trend occurring in the diencephalon and brainstem. NA was stable in the telencephalon but reached lower levels in the light and higher levels in the dark in the other two regions. In the telencephalon DA reached high levels early in the light and in the dark phases, showing a biphasic variation. Of particular interest was the apparent lack of correlation between cyclic changes in the monoamine levels and the changes in hydroxylation rates. Rates of hydroxylation can be considered indicative of rates of monoamine synthesis. This observation is discussed in relation to feedback and other mechanisms regulating synthesis and release of monoamines.

Key words

Tyrosine and Tryptophan Hydroxylase NSD 1015 Time of Day 


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Copyright information

© Springer-Verlag 1975

Authors and Affiliations

  • Jean DiRaddo
    • 1
  • Carol Kellogg
    • 1
  1. 1.Department of PsychologyUniversity of RochesterRochesterUSA

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