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Provoked iris ischaemia in the rabbit

II. Histochemical localization of sodium fluorescein

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Albrecht von Graefes Archiv für klinische und experimentelle Ophthalmologie Aims and scope Submit manuscript

Summary

The distribution of injected sodium fluorescein in the iris and ciliary body of albino rabbits was studied in normal animals and in animals subjected to different surgical procedures. The study was performed with two techniques: whole-mount preparations and the paraffin-section method on freeze-dried eyes. Fluorescein distribution showed marked changes after surgical procedures as a result of reduced blood perfusion. In normal iris vessels no penetration of fluorescein was observed. The permeability barrier was impaired if there were ischaemic conditions in the anterior segment. The results were compared with clinical and iris angiographic studies.

Zusammenfassung

An Albinokaninchen wurden durch chirurgische Maßnahmen Störungen der Blutzirkulation im vorderen Augenabschnitt herbeigeführt. 6–7 Wochen später erfolgte nach Fluoresceininjektion Enukleation der Augen. Die fluorescenzmikroskopische Untersuchung wurde sowohl im Flachschnittpräparat als auch im Paraffinschnitt bei gefriergetrockneten Augen durchgeführt. Die Verteilung des Fluorescein zeigte ausgeprägte Veränderungen im Vergleich zu den mit gleicher Methode untersuchten Kontrolltieren. Unter ischämischen Bedingungen war die Undurchlässigkeit der Blut-Gewebeschranke aufgehoben. Die Ergebnisse werden mit denen klinischer und irisangiographischer Untersuchungen verglichen.

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Authors and Affiliations

  1. Clinical Institute for Experimental Ophthalmology, University of Bonn, Federal Republic of Germany

    Petja Vassileva, Miguel Kottow & Erich Weigelin

Authors
  1. Petja Vassileva
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  2. Miguel Kottow
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  3. Erich Weigelin
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Fellows of the Alexander von Humboldt-Stiftung.

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Vassileva, P., Kottow, M. & Weigelin, E. Provoked iris ischaemia in the rabbit. Albrecht von Graefes Arch. Klin. Ophthalmol. 197, 31–38 (1975). https://doi.org/10.1007/BF00506638

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  • DOI: https://doi.org/10.1007/BF00506638

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