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Morphological Changes in the Retina Under Conditions of Experimental In Vivo Regional Ischemia/Reperfusion

  • MORPHOLOGY AND PATHOMORPHOLOGY
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Bulletin of Experimental Biology and Medicine Aims and scope

Two models of retinal ischemia/reperfusion were developed in an experiment on rats and structural changes in eye tissues in the early and late postischemic periods were studied. Ischemia/reperfusion was modeled by elevation of intraocular pressure to 110 mm Hg for 30 min with air injection into the anterior chamber of the eye with a special device or subconjunctival administration of 0.2 ml 4×10–6 M endothelin-1. Morphological studies of the retina of enucleated eyes were performed in 3, 7, and 30 days after ischemia/reperfusion. In 3 days, signs of retinal ischemia were seen (retinal edema and ganglion cell damage). In the late post-ischemic period (30 days), atrophy of the outer nuclear and outer plexiform layer of the retina was observed in animals with retinal ischemia/reperfusion caused by intraocular pressure elevation and complete destruction of neuronal cells was found after administration of endothelin-1.

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Authors and Affiliations

  1. Helmholtz Moscow Research Institute of Eye Diseases, Ministry of Health of the Russian Federation, Moscow, Russia

    T. N. Kiseleva, A. V. Chudin, I. P. Khoroshilova-Maslova, A. I. Shchipanova, A. M. Maibogin & M. S. Zaitsev

Authors
  1. T. N. Kiseleva
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  2. A. V. Chudin
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  3. I. P. Khoroshilova-Maslova
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  4. A. I. Shchipanova
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  5. A. M. Maibogin
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  6. M. S. Zaitsev
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Corresponding author

Correspondence to T. N. Kiseleva.

Additional information

Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 167, No. 2, pp. 250-256, February, 2019

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Kiseleva, T.N., Chudin, A.V., Khoroshilova-Maslova, I.P. et al. Morphological Changes in the Retina Under Conditions of Experimental In Vivo Regional Ischemia/Reperfusion. Bull Exp Biol Med 167, 287–292 (2019). https://doi.org/10.1007/s10517-019-04511-2

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  • DOI: https://doi.org/10.1007/s10517-019-04511-2

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