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5-HT mediated GABA excitatory responses in the guinea-pig proximal ileum

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Summary

  1. 1.

    GABA (3–100 μM) and 5-HT (0.03–30 μM) caused concentration-dependent transient contractions of the longitudinal muscle of the guinea-pig ileum.

  2. 2.

    The contractile response to GABA was antagonized by hyoscine (2.2 μM), TTX (0.7 μM), bicuculline (3 μM), furosemide (25 μM) and desensitization to GABA itself, while hexamethonium (20 μM) and methysergide (20 μM) were without effect.

  3. 3.

    The contractile response to 5-HT was antagonized by hyoscine (2.2 μM), TTX (0.7 μM) and desensitization to 5-HT itself and was unaffected by bicuculline (10 μM), hexamethonium (20 μM), furosemide (25 μM) and methysergide (20 μM).

  4. 4.

    A desensitization procedure that caused a 84.7-fold increase in the 5-HT EC50 also resulted in a 74.1-fold increase of the GABA EC50. Desensitization to GABA caused a reduction of 5-HT induced response but only in preparations desensitized by high (50 μM) concentrations of GABA.

  5. 5.

    The results indicate that GABA-induced contractions in the guinea-pig ileum are mediated by activation of cholinergic motor neurones. This effect appears to be mediated by interneuronal release of 5-HT rather than by a direct stimulatory action of GABA on the effector neurones.

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Authors and Affiliations

  1. Institute of Medical Pharmacology, University of Pavia, Piazza Botta 10, I-27100, Pavia, Italy

    M. Tonini, S. Lecchini, G. M. Frigo, E. Perucca & A. Crema

  2. Medical Clinic, University of L'Aquila, Viale Duca degli Abruzzi 3/A, I-67100, L'Aquila, Italy

    L. Onori & P. Saltarelli

Authors
  1. M. Tonini
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  2. L. Onori
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  3. S. Lecchini
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  4. G. M. Frigo
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  5. E. Perucca
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  6. P. Saltarelli
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  7. A. Crema
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Tonini, M., Onori, L., Lecchini, S. et al. 5-HT mediated GABA excitatory responses in the guinea-pig proximal ileum. Naunyn-Schmiedeberg's Arch. Pharmacol. 324, 180–184 (1983). https://doi.org/10.1007/BF00503891

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  • DOI: https://doi.org/10.1007/BF00503891

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