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The proximal gastric corpus is the most responsive site of motilin-induced contractions in the stomach of the Asian house shrew

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Abstract

The migrating motor complex (MMC) is responsible for emptying the stomach during the interdigestive period, in preparation for the next meal. It is known that gastric phase III of MMC starts from the proximal stomach and propagates the contraction downwards. We hypothesized that a certain region of the stomach must be more responsive to motilin than others, and that motilin-induced strong gastric contractions propagate from that site. Stomachs of the Suncus or Asian house shrew, a small insectivorous mammal, were dissected and the fundus, proximal corpus, distal corpus, and antrum were examined to study the effect of motilin using an organ bath experiment. Motilin-induced contractions differed in different parts of the stomach. Only the proximal corpus induced gastric contraction even at motilin 10−10 M, and strong contraction was induced by motilin 10−9 M in all parts of the stomach. The GPR38 mRNA expression was also higher in the proximal corpus than in the other sections, and the lowest expression was observed in the antrum. GPR38 mRNA expression varied with low expression in the mucosal layer and high expression in the muscle layer. Additionally, motilin-induced contractions in each dissected part of the stomach were inhibited by tetrodotoxin and atropine pretreatment. These results suggest that motilin reactivity is not consistent throughout the stomach, and an area of the proximal corpus including the cardia is the most sensitive to motilin.

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Authors and Affiliations

  1. Area of Regulatory Biology, Division of Life Science, Graduate School of Science and Engineering, Saitama University, 255 Shimo-okubo, Sakura-ku, Saitama, 338-8570, Japan

    Amrita Dudani, Gong Zhi, Ichiro Sakata & Takafumi Sakai

  2. Graduate School of Natural Science and Technology, Okayama University, 3-1-1 Tsushimanaka, Kita-ku, Okayama, 700-8530, Japan

    Sayaka Aizawa

  3. Department of Pharmaceutical and Health Sciences, Faculty of Pharmaceutical Sciences, Josai University, 1-1 Keiyaki dai, Sakado, Saitama, 350-0295, Japan

    Toru Tanaka

  4. Laboratory of Animal Management and Resources, Department of Zoology, Faculty of Science, Okayama University of Science, 1-1 Ridaicho, Kita-ku, Okayama, 700-0005, Japan

    Takamichi Jogahara

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  1. Amrita Dudani
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  2. Sayaka Aizawa
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  3. Gong Zhi
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  4. Toru Tanaka
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  6. Ichiro Sakata
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  7. Takafumi Sakai
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Correspondence to Takafumi Sakai.

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Communicated by I.D. Hume.

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Dudani, A., Aizawa, S., Zhi, G. et al. The proximal gastric corpus is the most responsive site of motilin-induced contractions in the stomach of the Asian house shrew. J Comp Physiol B 186, 665–675 (2016). https://doi.org/10.1007/s00360-016-0985-1

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  • DOI: https://doi.org/10.1007/s00360-016-0985-1

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