Abstract
The genetic control of NADH dehydrogenase-1 (NDH-1) and aromatic alcohol dehydrogenase-2 (AADH-2) was investigated in Triticum aestivum cv. Chinese Spring. Evidence was obtained that NDH-1 is active as a monomer and is encoded by genes located in the p arms of the homoeologous group 4 chromosomes. The NDH-1 gene loci located in 4 Ap, 4Bp, and 4Dp were designated Ndh-A1, Ndh-B1, and Ndh-D1, respectively. Aadh-A2 was previously reported to be located in 6Aq; in this study, Aadh-B2 and Aadh-D2 were localized in 6Bq and 6Dq, respectively. Alcohol dehydrogenase-1 is expressed on AADH-2 zymograms; the presence of a contaminating aliphatic alcohol in one or more reagents is suggested as the probable cause of this phenomenon.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Ainsworth, C. C., and Gale, M. D. (1987). Enzyme structural genes and their exploitation in wheat genetics and breeding. In Kruger, J. E., Lineback, D., and Stauffer, C. E. (eds.), Enzymes and Their Role in Cereal Technology Am. Assoc. Cereal Chem., St. Paul, Minn., pp. 53–82.
Beutler, E. (1967). “Galactose dehydrogenase,” “nothing dehydrogenase,” and alcohol dehydrogenase: Interrelation. Science 1561516.
Brown, A. H. D. (1983). Barley. In Tanksley, S. D., and Orton, T. J. (eds.), Isozymes in Plant Genetics and Breeding, Part B Elsevier, Amsterdam, pp. 57–77.
Brown, A. H. D., and Munday, J. (1981). Population-genetic structure and optimal sampling of land races of barley from Iran. Genetica 5885.
Hart, G. E. (1970). Evidence for triplicate genes for alcohol dehydrogenase in hexaploid wheat. Proc. Natl. Acad. Sci. USA 661136.
Hart, G. E. (1975). Glutamate oxaloacetate transaminase isozymes of Triticum: Evidence for multiple systems of triplicate structural genes in hexaploid wheat. In Market, C. (ed.), Isozymes, III Academic Press, New York, pp. 637–657.
Hart, G. E. (1979). Genetical and chromosomal relationships among the wheats and their relatives. Stadler Symp. Vol. 11, pp. 9–30.
Hart, G. E. (1983a). Hexaploid wheat (Triticum aestivum L. em Thell). In Tanksley, S. D., and Orton, T. J. (eds.), Isozymes in Plant Genetics and Breeding, Part B Elsevier, Amsterdam, pp. 35–56.
Hart, G. E. (1983b). Discovery and genetic control of a hexaploid wheat NAD-dependent alcohol dehydrogenase which acts on aromatic alcohols. Abstr. Bot. Soc. Am., p. 63.
Hart, G. E. (1983c). Genetics and evolution of multilocus isozymes in hexaploid wheat. In Ratazzi, M. C., Scandalios, J. G., and Whitt, G. S. (eds.), Isozymes: Current Topics in Biological and Medical Research, Vol. 10 Alan R. Liss, New York, pp. 365–380.
Hart, G. E. (1984). Biochemical loci of hexaploid wheat (Triticum aestivum, 2n=42, genomes AABBDD). In O'Brien, S. J. (ed.), Genetic Maps, Vol. 3 Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y., pp. 485–490.
Hart, G. E. (1987). Genetic and biochemical studies of enzymes. In Heyne, E. G. (ed.), Wheat and Wheat Improvement 2nd ed. American Society of Agronomy, Madison, Wis. pp. 199–214.
Hart, G. E., and Gale, M. D. (1987). Biochemical/molecular loci of hexaploid wheat (Triticum aestivum, 2n = 42, genomes AABBDD). In O'Brien, S. J. (ed.), Genetic Maps, Vol. 4 Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y. pp. 678–684.
Hart, G. E., and Tuleen, N. A. (1983a). Introduction and characterization of alien genetic material. In Tanksley, S. D., and Orton, T. J. (eds.), Isozymes in Plant Genetics and Breeding, Part B Elsevier, Amsterdam, pp. 339–362.
Hart, G. E., and Tuleen, N. A. (1983b). Chromosomal locations of eleven Elytrigia elongata (= Agropyron elongatum) isozyme structural genes. Genet. Res. Cambr. 41181.
Jaaska, V. (1978). NADP-dependent aromatic alcohol dehydrogenase in polyploid wheats and their diploid relatives. On the origin and phylogeny of polyploid wheats. Theor. Appl. Genet. 53209.
Jaaska, V. (1984). NAD-dependent aromatic alcohol dehydrogenase in wheats (Triticum L.) and goatgrasses (Aegilops L.): evolutionary genetics. Theor. Appl. Genet. 67535.
Jaaska, V. K., and Jaaska, V. E. (1978). Electrophoretic analysis of the substrate specificity of wheat alcohol dehydrogenases. Biochemistry (USSR) 431579.
McIntosh, R. A. (1986). Catalogue of gene symbols for wheat, 1986 supplement. In Quick, J. S. (ed.), Annual Wheat Newsletter, Vol. 32 Colorado State University, Ft. Collins, pp. 202–210.
Pearse, A. G. E. (1961). Histochemistry Little, Boston, Mass., p. 559.
Schmidt, J.-C., and Seliger, P. (1982). Nachweis von multiplen formen der alkoholdehydrogenase in blattmaterial von Triticum aestivum L. Carola. Biochem. Physiol. Pflanz. 177541.
Shaw, C. R., and Koen, A. L. (1965). On the identity of “nothing dehydrogenase.” J. Histochem. Cytochem. 13431.
Shaw, C. R., and Koen, A. L. (1967). “Galactose dehydrogenase,” “nothing dehydrogenase,” and alcohol dehydrogenase: Interrelation. Science 1561517.
Author information
Authors and Affiliations
Additional information
Technical article No. 22864 of the Texas Agricultural Experiment Station. This paper is based upon research supported in part by the U.S. Department of Agriculture under Agreement No. 83-CRCR-1-1322.
Rights and permissions
About this article
Cite this article
Hart, G.E. Genetic control of NADH dehydrogenase-1 and aromatic alcohol dehydrogenase-2 in hexaploid wheat. Biochem Genet 25, 837–846 (1987). https://doi.org/10.1007/BF00502603
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00502603