Summary
The effect of chlorpromazine (10 mg/kg i.v.) on the spontaneous release of endogenous dopamine (DA), noradrenaline (NA) and acetylcholine (ACh) within limbic areas perfused by means of the push-pull cannula was investigated in the gallamine-immobilized cat. Chlorpromazine increased the liberation of DA and NA in the nucleus accumbens septi, indicating blockade of the amine receptors. However, the drug did not change the output of ACh from this nucleus nor from ventral and dorsal hippocampal formation which receive a cholinergic input from the septum as indicated by several published findings and by the increased liberation of ACh after electrical stimulation of the homolateral nucleus medialis septi. These results seem to exclude that cholinergic neurons of some limbic areas mediate the effect of the blockade of DA (and of NA) receptors which is possibly involved in the antipsychotic action of neuroleptic drugs.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Andén, N. E.: Dopamine turnover in the corpus striatum and the limbic system after treatment with neuroleptic and antiacetylcholine drugs. J. Pharm. Pharmacol. 24, 905–906 (1972)
Bartholini, G., Lloyd, K. G., Stadler, H.: Dopaminergic regulation of cholinergic neurons in the striatum: relation to Parkinsonism. In: Princetown Conference on Parkinson Disease. F. McDowell and A. Barbeau, eds., Advances in Neurology, Vol. 5, pp. 11–14. London: Raven Press 1973
Bartholini, G., Stadler, H., Gadea Ciria, M., Lloyd, K. G.: The effect of antipsychotic drugs on the release of neurotransmitters in various brain areas. In: Antipsychotic drugs, Pharmacodynamics and Pharmacokinetics, Wenner-Gren Center International Symposium, Stockholm, Sept. 17–19, 1974. Oxford-London: Pergamon Press (in press)
Carlsson, A., Lindqvist, M.: Effect of chlorpromazine or haloperidol on formation of 3-methoxytyramine and normethanephrinein mouse brain. Acta pharmacol. (Kbh.) 20, 140–144 (1963)
Coyle, J. T., Henry, D.: Catecholamines in foetal and newborn rat brain. J. Neurochem. 21, 61–67 (1963)
Dudar, J. D.: The effect of septal nuclei stimulation on the release of acetylcholine from the rabbit hippocampus. Brain Res. 83, 123–133 (1975)
Guyenet, P., Agid, Y., Javoy, F., Beaujouan, J. C., Glowinski, J.: Action sélective des neuroleptiques sur les neurons cholinergiques du néo-striatum chez le rat: antagonisme vis-à-vis de l'apomorphine. C. R. Acad. Sci. (Paris) 278, 2679–2682 (1974)
Guyenet, P., Agid, Y., Javoy, F., Beaujouan, J. C., Rossier, J., Glowinski, J. Effects of dopaminergic receptor agonists and antagonists on the activity of the neo-striatal cholinergic system. Brain Res. 84, 227–244 (1975)
Ibata, Y., Desiraj, T., Pappas, G. D.: Light and electron microscopic study of the projection of the medial septal nucleus to the hippocampus of the cat. Exp. Neurol. 33, 103–122 (1971)
Ladinski, H., Consolo, S., Carattini, S.: Increase in striatal acetylcholine levels in vivo by piribedil, a new dopamine receptor stimulant. Life Sci. 14, 1251–1260 (1974)
Lloyd, K. G., Stadler, H., Bartholini, G.: Dopamine and acetylcholine neurons in striatal and limbic structures: effect of neuroleptic drugs. Frontiers in Catecholamine Research, E. Usdin and S. Snyder, eds. Oxford-London: Pergamon Press 1973
McGeer, E. G., Wada, J. A., Terao, A., Jung, E.: Amine synthesis in various regions with caudate or septal lesions. Exp. Neurol. 24, 277–284 (1969)
McGeer, P. L., Grewaal, D. S., McGeer, E. G.: Influence of non-cholinergic drugs on rat striatal acetylcholine levels. Brain Res. 89, 211–217 (1974)
Saelens, J. K., Allen, M. P., Simke, J. P.: Determination of acetylcholine and choline by an enzymatic assay. Arch. int. Pharmacodyn. 186, 279–286 (1970)
Schaffner, R., Jalfre, M., Haefely, W.: Effect of different pharmacological agents on the septo-hippocampal evoked potentials. CINP Congress, Paris, July 7–12, 1974 (Abstract C J 4)
Sethy, V. H., Roth, R. H., Kuhar, M. J., Van Woert, M. H.: Choline and acetylcholine: regional distribution and effect of degeneration of cholinergic nerve terminals in the rat hippocampus. Neuropharmacology 12, 819–823 (1973)
Sethy, V. H., Van Woert, M. H.: Modification of stratial acetylcholine concentration by dopamine receptor agonists and antagonists. Res. Comm. Chem. Path. Pharmacol. 1, 13–18 (1974)
Shute, C. C. D., Lewis, P. R.: The ascending cholinergic reticular system: neocortical, olfactory and subcortical projections. Brain 90, 497–520 (1967)
Smith, C. M.: Acetycholine release from the cholinergic septo-hippocampal pathway. Life Sci. 14, 2159–2166 (1974)
Snider, R. S., Niemer, W. T.: A stereotaxic atlas of the cat brain. Chicago: Univ. of Chicago Press 1961
Stadler, H., Lloyd, K. G., Bartholini, G.: Dopaminergic inhibition of striatal cholinergic neurons: Synergistic blocking action of γ-butyrolactone and neuroleptic drugs. Naunyn-Schmiedeberg's Arch. Pharmacol. 283, 129–134 (1974)
Stadler, H., Lloyd, K. G., Gadea Ciria, M., Bartholini, G.: Enhanced striatal acetylcholine release by chlorpromazine and its reversal by apomorphine. Brain. Res. 55, 476–480 (1973)
Trabucchi, M., Cheney, D., Racagni, G., Costa, E.: Involvement of brain cholinergic mechanisms in the action of chlorpromazine. Nature (Lond.) 249, 664–666 (1974)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Stadler, H., Ciria, M.G. & Bartholini, G. “In vivo” release of endogenous neurotransmitters in cat limbic regions: Effect of chlorpromazine and of electrical stimulation. Naunyn-Schmiedeberg's Arch. Pharmacol. 288, 1–6 (1975). https://doi.org/10.1007/BF00501809
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00501809